Evaluating peripheral blood lymphocyte subset composition and lipopolysaccharide-induced cytokine secretory activity in mononuclear leukocyte cultures from patients with febrile and meningeal forms of tick-borne encephalitis
- 作者: Ilyinskikh E.N.1, Voronkova O.V.1, Reshetova A.V.1, Hasanova R.R.1, Esimova I.E.1, Chernyshov N.A.1, Yampolskaya O.V.1, Yampolskaya A.V.1, Polomoshnova E.M.1, Minakova E.V.1
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隶属关系:
- Siberian State Medical University
- 期: 卷 14, 编号 4 (2024)
- 页面: 690-700
- 栏目: ORIGINAL ARTICLES
- URL: https://journals.rcsi.science/2220-7619/article/view/268702
- DOI: https://doi.org/10.15789/2220-7619-EPB-17647
- ID: 268702
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Introduction. Multiple studies on immune response in patients with various clinical forms of tick-borne encephalitis (TBE) have shown conflicting results. The study aim was to estimate the changes in peripheral blood lymphocyte subset counts and activity of spontaneous and lipopolysaccharide (LPS)-stimulated cytokine production in the mononuclear leukocyte cultures in patients with febrile and meningeal acute TBE. Materials and methods. Groups 1 and 2 included 16 and 12 patients with febrile and meningeal acute TBE, respectively. The control group included 13 healthy donors. Hemogram and T-lymphocyte, T-helper cell, T-cytotoxic and NK cell counts were analyzed by flow cytometry at week 1 of the disease as well as the spontaneous and LPS-stimulated secretion levels of TNFα, IL-1β, IL-6, IL-10, IL-8, and MCP-1 were assessed by ELISA in the supernatants of mononuclear cell cultures twice: during hospitalization and two weeks later. Statistical analysis was performed by the Mann–Whitney U-test, and Wilcoxon test. Results. Group 2 demonstrated significant decrease in spontaneous and/or LPS-stimulated levels of proinflammatory cytokines IL-1β, IL-6, MCP-1, and TNFα, but showed higher IL-8 level as compared with Group 1. In addition, spontaneous and/or LPS-induced levels of IL-6 and TNFα in Group 2 did not significantly differ from the controls, which presumably also indicated the suppression of their production. In contrast, spontaneous and/or LPS-induced levels of IL-1β, IL-6, MCP-1, and TNFα in Group 1 were higher than in the controls. The spontaneous IL-10 level in the patients from both groups were higher than in the controls. Peripheral blood lymphocyte and T-cytotoxic T-lymphocyte counts in both groups of TBE patients were lower than in the controls. There was significant increase in neutrophil counts, decrease in NK cell count in Group 2 as compared to the patients with a milder febrile form. Conclusion. Meningeal acute TBE patients was presumably associated with inadequate immune response with NK cell deficiency, T-cell dysfunction, increased neutrophil count in the peripheral blood and impaired pro-inflammatory cytokine production related to innate immune response.
作者简介
Ekaterina Ilyinskikh
Siberian State Medical University
编辑信件的主要联系方式.
Email: infconf2009@mail.ru
д.м.н., доцент, профессор кафедры инфекционных болезней и эпидемиологии
俄罗斯联邦, TomskO. Voronkova
Siberian State Medical University
Email: infconf2009@mail.ru
DSc (Medicine), Associate Professor, Head of the Division of Biology and Genetics
俄罗斯联邦, TomskA. Reshetova
Siberian State Medical University
Email: infconf2009@mail.ru
PhD Student of the Division of Infectious Diseases and Epidemiology
俄罗斯联邦, TomskR. Hasanova
Siberian State Medical University
Email: infconf2009@mail.ru
PhD (Medicine), Associate Professor of the Division of Biology and Genetics
俄罗斯联邦, TomskI. Esimova
Siberian State Medical University
Email: infconf2009@mail.ru
DSc (Medicine), Associate Professor of the Division of Biology and Genetics
俄罗斯联邦, TomskN. Chernyshov
Siberian State Medical University
Email: infconf2009@mail.ru
PhD Student of the Division of Biology and Genetics
俄罗斯联邦, TomskO. Yampolskaya
Siberian State Medical University
Email: infconf2009@mail.ru
Student
俄罗斯联邦, TomskA. Yampolskaya
Siberian State Medical University
Email: infconf2009@mail.ru
Student
俄罗斯联邦, TomskE. Polomoshnova
Siberian State Medical University
Email: infconf2009@mail.ru
Student
俄罗斯联邦, TomskE. Minakova
Siberian State Medical University
Email: infconf2009@mail.ru
Student
俄罗斯联邦, Tomsk参考
- Аитов К.А., Бурданова Т.М., Верхозина М.М., Демина Т.В., Джиоев Ю.П., Козлова И.В., Лемешевская М.В., Малов С.И., Орлова Л.С., Ткачев С.Е., Малов И.В., Злобин В.И. Клещевой энцефалит в Восточной Сибири: этиология, молекулярная эпидемиология, особенности клинического течения // Инфекционные болезни: новости, мнения, обучение. 2018. Т. 7, № 3 (26). С. 31–40. [Aitov K.A., Burdanova T.M., Verkhozina M.M., Demina T.V., Dzhioev Yu.P., Kozlova I.V., Lemeshevsckaya M.V., Malov S.I., Orlova L.S., Tkachev S.E., Malov I.V., Zlobin V.I. Tick-borne encephalitis in Eastern Siberia: etiology, molecular epidemiology and peculiarities of the clinical course. Infekcionne bolezni: novosti, mnenija, obuchenie = Infectious Diseases: News, Opinions, Training, 2018, vol. 7, no. 3 (26), pp. 31–40. (In Russ.)] doi: 10.24411/2305-3496-2018-13005
- Иерусалимский А.П. Клещевой энцефалит: руководство для врачей. Новосибирск: Государственная медицинская академия МЗ РФ, 2001. 360 с. [Ierusalimsky A.P. Tick-borne encephalitis. Manual for physicians. Novosibirsk: State Medical Academy Publishers, 2001. 360 p. (In Russ.)]
- Крылова Н.В., Леонова Г.Н., Павленко Е.В., Запорожец Т.С., Смолина Т.П., Гажа А.К., Новиков Д.В., Ченцова И.В. Комплексная оценка состояния иммунной системы при различных формах клещевого энцефалита в остром периоде // Медицинская иммунология. 2012. Т. 14, № 4–5. С. 313–320. [Krylova N.V., Leonova G.N., Pavlenko E.V., Zaporozhets T.S., Smolina T.P., Gazha A.K., Novikov D.V., Chenzova I.V. Comprehensive assessment of immune system in various forms of tick-borne encephalitis in acute phase. Meditsinskaya immunologiya = Medical Immunology (Russia), 2012, vol. 14, no. 4–5, pp. 313–320. (In Russ.)] doi: 10.15789/1563-0625-2012-4-5-313-320
- Мерфи К., Уивер К. Иммунобиология по Джанвэю. Под ред. Г.А. Игнатьевой, О.А. Свитич, И.Н. Дьякова. М.: Логосфера, 2020. 1184 с. [Murphy K., Weaver C. Janeway’s Immunology. Eds.: Ignat’eva G.A., Svitich O.A., D’yakova I.N. Moscow: Logosfera, 2020. 1184 p. (In Russ.)]
- Петри А., Сэбин К. Наглядная медицинская статистика. Под ред. В.П. Леонова. М.: ГЭОТАР-Медиа, 2021. 232 с. [Petrie A., Sabin K. Medical statistics at a glance. Ed. Leonov V.P. Moscow: GEOTAR-Media, 2021. 232 p. (In Russ.)]
- Стенько Е.А., Ратникова Л.И., Ермакова Н.В. Состояние клеточного и гуморального иммунитета при инфицировании вирусом семейства Flaviviridae // Известия высших учебных заведений. Уральский регион. 2013. № 2. С. 138–144. [Stenko E.A., Ratnikova L.I., Ermakova N.V. Cellular and humoral immunity when infected by a virus of the family Flaviviridae. Izvestiya vysshikh uchebnykh zavedeniy. Ural’skiy region = News of Higher Educational Institutions. Ural Region, 2013, no. 2, pp. 138–144. (In Russ.)]
- Сумливая О.Н., Воробьева Н.Н., Каракулова Ю.В. Диагностическое значение определения концентрации серотонина и высокочувствительного С-реактивного белка в крови у больных клещевым энцефалитом // Медицинская паразитология и паразитарные болезни. 2014. № 2. С. 25–29. [Sumlivaya O.N., Vorobyova N.N., Karakulova Yu.V. Diagnostic value of determining the concentration of serotonin and high-sensitivity C-reactive protein in the blood in patients with tick-borne encephalitis. Meditsinskaia parazitologiia i parazitarnye bolezni = Medical Parasitology and Parasitic Diseases, 2014, no. 2, pp. 25–29. (In Russ.)]
- Arunachalam P.S., Wimmers F., Mok C.K.P., Perera R.A.P.M., Scott M., Hagan T., Sigal N., Feng Y., Bristow L., Tak-Yin Tsang O., Wagh D., Coller J., Pellegrini K.L., Kazmin D., Alaaeddine G., Leung W.S., Chan J.M.C., Chik T.S.H., Choi C.Y.C., Huerta C., Paine McCullough M., Lv H., Anderson E., Edupuganti S., Upadhyay A.A., Bosinger S.E., Maecker H.T., Khatri P., Rouphael N., Peiris M., Pulendran B. Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans. Science, 2020, vol. 369, no. 6508, pp. 1210–1220. doi: 10.1126/science.abc6261
- Björkström N.K., Strunz B., Ljunggren H.G. Natural killer cells in antiviral immunity. Nat. Rev. Immunol., 2022, vol. 22, no. 2, pp. 112–123. doi: 10.1038/s41577-021-00558-3
- Blom K., Braun M., Pakalniene J., Lunemann S., Enqvist M., Dailidyte L., Schaffer M., Lindquist L., Mickiene A., Michaëlsson J., Ljunggren H.G., Gredmark-Russ S. NK Cell responses to human tick-borne encephalitis virus infection. J. Immunol., 2016, vol. 197, no. 7, pp. 2762–2771. doi: 10.4049/jimmunol.1600950
- Blom K., Cuapio A., Sandberg J.T., Varnaite R., Michaëlsson J., Björkström N.K., Sandberg J.K., Klingström J., Lindquist L., Gredmark Russ S., Ljunggren H.G. Cell-mediated immune responses and immunopathogenesis of human tick-borne encephalitis virus-infection. Front. Immunol., 2018, no. 9: 2174. doi: 10.3389/fimmu.2018.02174
- Bogovič P., Kastrin A., Lotrič-Furlan S., Ogrinc K., Avšič Županc T., Korva M., Knap N., Resman Rus K., Strle K., Strle F. Comparison of laboratory and immune characteristics of the initial and second phase of tick-borne encephalitis. Emerg. Microbes Infect., 2022, vol. 11, no. 1, pp. 1647–1656. doi: 10.1080/22221751.2022.2086070
- Bogovič P., Lusa L., Korva M., Lotrič-Furlan S., Resman-Rus K., Pavletič M., Avšič-Županc T., Strle K., Strle F. Inflammatory immune responses in patients with tick-borne encephalitis: dynamics and association with the outcome of the disease. Microorganisms, 2019, vol. 7, no. 11:. 514. doi: 10.3390/microorganisms7110514
- Ciesielska A., Matyjek M., Kwiatkowska K. TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cell. Mol. Life Sci., 2021, vol. 78, no. 4, pp. 1233–1261. doi: 10.1007/s00018-020-03656-y
- Gerada C., Campbell T.M., Kennedy J.J., McSharry B.P., Steain M., Slobedman B., Abendroth A. Manipulation of the innate immune response by varicella zoster virus. Front. Immunol., 2020, no. 11: 1. doi: 10.3389/fimmu.2020.00001
- Krylova N.V., Smolina T.P., Leonova G.N. Molecular mechanisms of interaction between human immune cells and Far Eastern tick-borne encephalitis virus strains. Viral Immunol., 2015, vol. 28, no. 5, pp. 272–281. doi: 10.1089/vim.2014.0083
- Lannoy V., Côté-Biron A., Asselin C., Rivard N. TIRAP, TRAM, and Toll-Like receptors: the untold story. Mediators Inflamm., 2023: 2899271. doi: 10.1155/2023/2899271
- Lindqvist R., Upadhyay A., Överby A.K. Tick-borne Flaviviruses and the type I interferon response. Viruses, 2018, vol. 10, no. 7: 340. doi: 10.3390/v10070340
- Ma N., Li X., Jiang H., Dai Y., Xu G., Zhang Z. Influenza virus neuraminidase engages CD83 and promotes pulmonary injury. J. Virol., 2021, vol. 95, no. 3: e01753-20. doi: 10.1128/JVI.01753-20
- Mathew A. Defining the role of NK cells during dengue virus infection. Immunology, 2018, vol. 154, no. 4, pp. 557–562. doi: 10.1111/imm.12928
- Sekaran S.D., Ismail A.A., Thergarajan G., Chandramathi S., Rahman S.K.H., Mani R.R., Jusof F.F., Lim Y.A.L., Manikam R. Host immune response against DENV and ZIKV infections. Front. Cell. Infect. Microbiol., 2022, no. 12: 975222. doi: 10.3389/fcimb.2022.975222
- Sharma K.B., Chhabra S., Kalia M. Japanese encephalitis virus-infected cells. Subcell. Biochem., 2023, no. 106, pp. 251–281. doi: 10.1007/978-3-031-40086-5_10
- Zidovec-Lepej S., Vilibic-Cavlek T., Ilic M., Gorenec L., Grgic I., Bogdanic M., Radmanic L., Ferenc T., Sabadi D., Savic V., Hruskar Z., Svitek L., Stevanovic V., Peric L., Lisnjic D., Lakoseljac D., Roncevic D., Barbic L. Quantification of antiviral cytokines in serum, cerebrospinal fluid and urine of patients with tick-borne encephalitis in Croatia. Vaccines (Basel), 2022, vol. 10, no. 11: 1825. doi: 10.3390/vaccines10111825
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