Features of ESCHERICHIA COLI samples from patients with diarrheal syndrome in the Republic of Guinea

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Abstract

Introduction. Diarrheal diseases are a global public health issue and cause 15% of deaths in children under 5 years old, of which about 80% occur in the regions of Africa and Southeast Asia. According to the Global Enteric Multicentre Study (GEMS) conducted in a number of African countries, one of the leading pathogens of high risk of death in infants and young children is diarrheagenic E. coli (DEC). In recent decades, antimicrobial resistance (AMR) has become globally ubiquitous. The Republic of Guinea urgently needs large-scale studies devoted to assessing DEC distribution and antibiotic resistance. The purpose of the study is to assess the pattern of E. coli infections and to test the susceptibility to antibiotics in strains of diarrheagenic E. coli sampled from individuals residing in the Republic of Guinea.

Materials and methods. From 2019 to 2022, we studied 724 samples of faeces of patients with acute diarrhea, among them 72 (9.9%) children aged 1–5 years, 128 (17.7%) children aged 6–17 years, and 524 (72.4%) people aged 18 years and older; a method of polymerase chain reaction (PCR) was applied with the use of the AmpliSense® Escherichioses-FL reagent kit to identify the genetic determinants of DEC: EPEC, EHEC, ETEC, EIEC, and EAgEC (Central Research Institute of Epidemiology of Rospotrebnadzor, Russia). Susceptibility to 15 antimicrobial agents was found by the disc-diffusion method using Mueller–Hinton agar (Russia) and Oxoid discs (UK). Results were interpreted according EUCAST criteria, versions 2019–2022 (https://www.eucast.org/ast_of_bacteria/previous_versions_of_documents).

Results. For the period from 2019 to 2022, the percentage of E. coli infections in the etiological pattern of acute intestinal infections amounted to 51.7%. In the age-related manner, DEC was significantly more common in young children aged 0–5 (96.9%, p < 0.05) as compared to school age children aged 6–17 (53.9%) and adults (45.6%). In all years of observation, EAgEC strains prevailed, accounting for 38.4%. Other DEC pathotypes, EPEC, ETEC, EIEC and STEC, accounted for 27.2%, 17.5%, 11.8%, and 5.1%, respectively. DEC strains are susceptible to meropenem, amikacin, and nitrofurantoin. The activity of other antibiotics ranged from 11.3% for ampicillin, 28.3% for trimethoprim-sulfamethoxazole, and 34.0% for tetracycline to 73.6% for cephalosporins, 84.0% for aminoglycosides, and 98.1% for fluorinated quinolones.

Conclusion. To reduce the burden of diarrheal diseases in the Republic of Guinea, it may be necessary to conduct targeted epidemiological and microbiological studies to identify DEC and monitor the development of antimicrobial resistance of E. coli infection pathogens in the population.

About the authors

M. A. Makarova

St. Petersburg Pasteur Institute; I.I. Mechnikov North-Western State Medical University

Author for correspondence.
Email: makmaria@mail.ru

DSc (Medicine), Senior Researcher, Laboratory of Enteric Infections; Associate Professor, Department of Medical Microbiology

Russian Federation, Saint-Petersburg; Saint-Petersburg

R. Balde

Research Institute of Applied Biology of Guinea

Email: makmaria@mail.ru

Researcher, Department of Bacteriology
Guinea, Kindia

M. Boiro

Research Institute of Applied Biology of Guinea

Email: makmaria@mail.ru

Professor, General Director
Guinea, Kindia

Z. N. Matveeva

St. Petersburg Pasteur Institute

Email: makmaria@mail.ru

PhD (Medicine), Leading Researcher, Laboratory of Enteric Infections

Russian Federation, St. Petersburg

L. A. Kaftyreva

St. Petersburg Pasteur Institute; I.I. Mechnikov North-Western State Medical University

Email: makmaria@mail.ru

DSc (Medicine), Leading Researcher, Typhoid Epidemiology Research Group;  Professor, Department of Medical Microbiology

Russian Federation, Saint-Petersburg; Saint-Petersburg

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Supplementary files

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2. Figure 1. Structure of E. coli infections in the Republic of Guinea, 2019–2022

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3. Figure 2. Antimicrobial agent susceptibility in DEC strains

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Copyright (c) 2023 Makarova M.A., Balde R., Boiro M., Matveeva Z.N., Kaftyreva L.A.

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