Features of the Candida genus community pattern in the intestinal biotope of patients with tuberculosis
- Authors: Kholodov A.A.1, Zakharova Y.V.1, Otdushkina L.Y.1, Bryukhacheva E.O.1, Pyanzova T.V.1
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Affiliations:
- Kemerovo State Medical University of the Ministry of Health of Russia
- Issue: Vol 12, No 6 (2022)
- Pages: 1169-1174
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/2220-7619/article/view/119191
- DOI: https://doi.org/10.15789/2220-7619-FOT-1974
- ID: 119191
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Abstract
Fungi are opportunistic microorganisms that colonize all biotopes of the human body, including intestinal. In case of emerging adverse environmental factors (HIV infection, other immunodeficiencies, antibiotic therapy), these microbial representatives begin active reproduction, which might require prescribing antimycotics. Frequent use of the latter in clinical practice induces the development of drug resistance to antifungal drugs, which may impact on effectiveness of both the treatment of fungal infections and other diseases. The purpose of the study was to assess the pattern and spectrum of drug resistance of Candida genus in the intestinal biotope of patients with respiratory tuberculosis and identify risk factors for developing total fungal drug resistance to antimycotic drugs. Material and methods. There were enrolled 21 patients with respiratory tuberculosis. Pattern of the fungal species diversity for the Candida genus isolated from faeces was evaluated, and the spectrum of relevant drug resistance to antimycotic drugs was determined. Patients (n = 21) were divided into 2 groups: with (n = 10) and without (n = 11) total resistance to antimycotics, after which the main risk factors for its development were identified. Results. Members of the Candida genus were isolated from all patients examined, wherein pure cell cultures were characterized by high level of antimycotics resistance. Resistance to three drugs was noted in 1 culture (4.8%), to four — in 10 cultures (47.6%), also found in 10 cultures to the entire drug panel (47.6%). During statistical processing, the data were obtained on affecting formation of total resistance to antimycotics of concomitant pathology of the gastrointestinal tract, the presence of a clinically significant dyspeptic syndrome, a history of antimycotic therapy, HIV infection with severe immunodeficiency, and some decrease in the peripheral blood CD4+ lymphocyte count. Conclusion. Fungi of the Candida genus isolated from tuberculosis patients were characterized by high level of resistance to antimycotics. Total resistance was observed in 47.6% of patients. In addition, the major fungi colonizing the intestines of tuberculosis patients were found to be Candida albicans species. The risk factors for the development of total antifungal resistance included: chronic enterocolitis, dyspeptic syndrome, peripheral blood CD4+ lymphocyte count lower than 350 cells/µl, and history of antimycotic therapy.
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##article.viewOnOriginalSite##About the authors
Artyom A. Kholodov
Kemerovo State Medical University of the Ministry of Health of Russia
Email: artyomkass96@gmail.com
ORCID iD: 0000-0001-5249-8822
Clinical Resident, Department of Phthisiology
Russian Federation, 650029, Kemerovo, Voroshilov str., 22AYu. V. Zakharova
Kemerovo State Medical University of the Ministry of Health of Russia
Email: artyomkass96@gmail.com
ORCID iD: 0000-0002-3475-9125
PhD, MD (Medicine), Associate Professor, Associate Professor of the Department of Microbiology
Russian Federation, 650029, Kemerovo, Voroshilov str., 22ALarisa Yu. Otdushkina
Kemerovo State Medical University of the Ministry of Health of Russia
Email: artyomkass96@gmail.com
ORCID iD: 0000-0003-4126-4312
Assistant Professor, Department of Microbiology, Immunology and Virology
Russian Federation, 650029, Kemerovo, Voroshilov str., 22AE. O. Bryukhacheva
Kemerovo State Medical University of the Ministry of Health of Russia
Email: artyomkass96@gmail.com
ORCID iD: 0000-0001-5212-9234
Assistant Professor, Department of Phthisiology
Russian Federation, 650029, Kemerovo, Voroshilov str., 22ATatiana V. Pyanzova
Kemerovo State Medical University of the Ministry of Health of Russia
Author for correspondence.
Email: artyomkass96@gmail.com
ORCID iD: 0000-0002-4854-5734
PhD, MD (Medicine), Associate Professor, Department of Phthisiology
Russian Federation, 650029, Kemerovo, Voroshilov str., 22AReferences
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