Age-Related Changes in the Content of Sirtuin 1 in Human Dermal Fibroblasts

Abstract

The goal of our work was to examine the localization and expression level of sirtuin 1 (SIRT1) in human dermal fibroblasts at different stages of ontogenesis, as well as to identify the role of SIRT1 in skin aging. The study was based on an autopsy skin material sampled from fetuses dying prenatally in the 20th to 40th week of gestation and from people dying in the period starting from birth to the age of 85 years. SIRT1 and the proliferating cell nuclear antigen (PCNA) were detected by the indirect immunohistochemical method. The results of our study have shown that the SIRT1 level decreases in dermal fibroblasts during the period from birth until the age of 85 and that the proliferative activity of dermal fibroblasts and their counts are steadily reduced. The decreasing content of SIRT1 in dermal fibroblasts with age correlates with a reduction of their proliferative activity and can be considered as one of the mechanisms for inhibition of the proliferation of dermal fibroblasts.