Characteristics of age-dependent changes in urine proteome in healthy men

Abstract

This paper studies the age dynamics of the proteomic profile of urine in healthy volunteers. The proteome composition was determined by chromatography-mass-spectrometry based on a nanostream highly efficient liquid chromatograph (Agilent 1100), and mass-spectra were obtained with a LTQ-FT hybrid mass-spectrometer. The urine samples obtained from 52 healthy men aged 19–54 years were found to contain 259 various proteins. According to the TiGER database, the tissue origin was established for 141 of them, and 715 biological processes in which they participate were identified. A significant positive correlation of the number (R = 0.566, p-value = 1.24E–05) and weight of proteins (R = 0.45; p-value = 8.17E–04) with age was found. We identified 23 proteins that are significantly more frequent in urine with increasing age of the subjects (p < 0.05) and only one protein, RGSL1, that is a regulator of signal transmission through receptors connected with G-protein (MW 125.69), which becomes less frequent with increasing age.