Lamin B1 and lamin B2 in human skin in the process of aging

Abstract

The goal of this work was to study type B lamins in human skin at different ages. Lamins B1 and B2 were detected in sections of the skin by indirect immunohistochemistry. There were 62.3% of dermal fibroblasts with positive staining for lamin B1 at the period from 20 to 40 weeks of gestation. From birth to 40 years, 41–42% of fibroblasts containing lamin B1 were found in the dermis. In the age interval from 41 to 85 years, 57–60% of dermal fibroblasts had positive staining for lamin B1. The number of fibroblasts containing lamin B2 was gradually decreasing from 80.6 to 68.6% for ages from 20 weeks of gestation to 85 years. The expression of lamin B1 in the nuclei of fibroblasts decreased from birth to 40 years. The lamin B2 content in fibroblast nuclei was almost constant from 20 weeks of gestation to 85 years. The total number of fibroblasts in the dermis diminished with age. The most significant decrease in the number of fibroblasts was observed for ages from 20 weeks of gestation to 20 years. The results make it possible to suggest the participation of lamin B1 in triggering the age-dependent decrease in the number of fibroblasts in the human dermis.