Immunomorphologic assessment of changes in functional astroglial proteins in a kainate-induced hippocampal sclerosis model
- 作者: Voronkov D.1, Egorova A.1,2, Fedorova E.1,2, Stavrovskaya A.1, Potapov I.1, Pavlova A.1, Sukhorukov V.1,2
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隶属关系:
- Research Center of Neurology
- Pirogov Russian National Research Medical University
- 期: 卷 18, 编号 2 (2024)
- 页面: 34-44
- 栏目: Original articles
- URL: https://journals.rcsi.science/2075-5473/article/view/262419
- DOI: https://doi.org/10.17816/ACEN.1102
- ID: 262419
如何引用文章
详细
Introduction. Astrocytes are involved in mediator metabolism, neuroplasticity, energy support of neurons and neuroinflammation, and this determines their pathogenetic role in epilepsy.
Aim. This study aimed at evaluating region-specific changes in the distribution of functional astroglial proteins in reactive astrocytes in a kainate-induced model of mesial temporal lobe epilepsy.
Materials and methods. The localization and expression of functional astroglial proteins (i.e. aquaporin-4, connexin-43, EAAT1/2, and glutamine synthetase) in the hippocampus CA3 region, dentate gyrus, and stratum lucidum layer were evaluated by immunofluorescence 28 days after intra-hippocampal administration of kainic acid to animals.
Results. Changes were heterogeneous in different hyppocampus subregions. Astrocytes of the stratum lucidum associated with mossy fibers showed the highest vulnerability and decreased content and/or disturbed localization of the channels and transporters that form membrane complexes in the processes. Disturbances in homeostatic functions of astrocytes aggravated the adverse processes both on the side where the toxin was injected and in the contralateral hippocampus.
作者简介
Dmitry Voronkov
Research Center of Neurology
编辑信件的主要联系方式.
Email: voronkov@neurology.ru
ORCID iD: 0000-0001-5222-5322
Cand. Sci. (Med.), senior researcher, Laboratory of neuromorphology, Brain Institute, Research Center of Neurology
俄罗斯联邦, MoscowAnna Egorova
Research Center of Neurology; Pirogov Russian National Research Medical University
Email: av_egorova@bk.ru
ORCID iD: 0000-0001-7112-2556
Cand. Sci. (Med.), researcher, Neuromorphology laboratory, Brain Institute, Research Center of Neurology; Associate Professor, Department of histology, embryology and cytology, Pirogov Russian National Research Medical University
俄罗斯联邦, Moscow; MoscowEvgenia Fedorova
Research Center of Neurology; Pirogov Russian National Research Medical University
Email: ewgenia.feodorowa2011@yandex.ru
ORCID iD: 0000-0002-2128-9056
research laboratory assistant, Neuromorphology laboratory, Brain Institute, Research Center of Neurology; assistant, Department of histology, embryology and cytology, Pirogov Russian National Research Medical University
俄罗斯联邦, Moscow; MoscowAlla Stavrovskaya
Research Center of Neurology
Email: stavrovskaya.al@gmail.com
ORCID iD: 0000-0002-8689-0934
Cand. Sci. (Biol.), Head, Laboratory of experimental pathology of the nervous system and neuropharmacology, Brain Institute, Research Center of Neurology
俄罗斯联邦, MoscowIvan Potapov
Research Center of Neurology
Email: potapov.i.a@neurology.ru
ORCID iD: 0000-0002-7471-3738
junior researcher, Laboratory of experimental pathology of the nervous system and neuropharmacology, Brain Institute, Research Center of Neurology
俄罗斯联邦, MoscowAnastasiya Pavlova
Research Center of Neurology
Email: pav_nastasya@mail.ru
ORCID iD: 0009-0006-5653-5524
research laboratory assistant, Laboratory of experimental pathology of the nervous system and neuropharmacology, Brain Institute, Research Center of Neurology
俄罗斯联邦, MoscowVladimir Sukhorukov
Research Center of Neurology; Pirogov Russian National Research Medical University
Email: sukhorukov@neurology.ru
ORCID iD: 0000-0002-0552-6939
D. Sci. (Med.), Head, Laboratory of neuromorphology, Brain Institute, Research Center of Neurology; Professor, Department of histology, embryology and cytology, Pirogov Russian National Research Medical University
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