The content of matrix metalloproteinase-9 in aqueous humor of the eyes in children with endogenous uveitis

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Abstract

AIM: This study aimed to determine the content of matrix metalloproteinase-9 (MMP-9) in the aqueous humor (AH) of the eyes in children with uveitis and its role during the disease.

MATERIAL AND METHODS: Twenty children (20 eyes with uveitis) aged from 3 to 16 yr (11.25±3.43 yr on average) and three children with congenital cataract from the control group were examined. The concentration of MMP-9 was determined by enzyme-linked immunosorbent assay (ELISA) using the ELISA kit for MMP-9 (Cloud-Clone Corp, USA). The optical density of the samples was measured using a multifunctional photometer for Synergy microplates (BioTek, USA).

RESULTS: Children with uveitis had a higher content of MMP-9 in the AH than children in the control group (p=0.006). An increase in the content of MMP-9 in the AH of the eyes was correlated with an increase in the degree of proliferative changes in the eye.

CONCLUSION: The concentration of MMP-9 in the AH of the eyes correlates with the severity of proliferation, and its measurement can be used to assess the severity of the inflammatory process in the eye.

About the authors

Lyudmila A. Katargina

Helmholtz National Medical Research Center of Eye Diseases

Email: katargina@igb.ru
ORCID iD: 0000-0002-4857-0374

MD, Dr. Sci. (Med.), Professor

Russian Federation, Moscow

Natalya B. Chesnokova

Helmholtz National Medical Research Center of Eye Diseases

Email: nchesnokova2012@yandex.ru
ORCID iD: 0000-0002-7856-8005
SPIN-code: 8705-7248

MD, Dr. Sci. (Biol.), Professor

Russian Federation, Moscow

Ekaterina V. Denisova

Helmholtz National Medical Research Center of Eye Diseases

Email: deale_2006@inbox.ru
ORCID iD: 0000-0003-3735-6249
SPIN-code: 4111-4330

MD, Cand. Sci. (Med.)

Russian Federation, Moscow

Maria A. Khrabrova

Helmholtz National Medical Research Center of Eye Diseases

Author for correspondence.
Email: khrabrovamaria@mail.ru
ORCID iD: 0000-0001-9422-4264

Graduate Student

Russian Federation, Moscow

Olga V. Beznos

Helmholtz National Medical Research Center of Eye Diseases

Email: olval2011@mail.ru
ORCID iD: 0000-0001-7557-4955
SPIN-code: 7894-5162

MD, Doctor

Russian Federation, Moscow

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2. Fig. 1. The content of matrix metalloproteinase-9 (MMP-9) in the aqueous humor (AH) of eyes with uveitis and in the control group.

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3. Fig. 2. The content of matrix metalloproteinase–9 (MMP-9) in the aqueous humor (AH) of eyes with varying degrees of proliferative changes.

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Copyright (c) 2022 Katargina L.A., Chesnokova N.B., Denisova E.V., Khrabrova M.A., Beznos O.V.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
 


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