Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

S. N. Tamkovich; N. V. Yunusova; M. N. Stakheeva; A. K. Somov; A. E. Frolova; N. A. Kiryushina; S. G. Afanasyev; A. E. Grigor’eva; P. P. Laktionov; I. V. Kondakova

doi:10.1134/S1990750817030106

Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients

作者: Tamkovich S.N.¹^,2, Yunusova N.V.³, Stakheeva M.N.³, Somov A.K.¹, Frolova A.E.³, Kiryushina N.A.⁴, Afanasyev S.G.³, Grigor’eva A.E.¹, Laktionov P.P.¹, Kondakova I.V.³
隶属关系:
1. Institute of Chemical Biology and Fundamental Medicine
2. Novosibirsk State University
3. Tomsk Cancer Research Institute
4. Novosibirsk Regional Oncological Dispensary
期: 卷 11, 编号 3 (2017)
页面: 291-295
栏目: Article
URL: https://journals.rcsi.science/1990-7508/article/view/197929
DOI: https://doi.org/10.1134/S1990750817030106
ID: 197929

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详细

A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.