Email this article Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients
- Authors: Tamkovich S.N.1,2, Yunusova N.V.3, Stakheeva M.N.3, Somov A.K.1, Frolova A.E.3, Kiryushina N.A.4, Afanasyev S.G.3, Grigor’eva A.E.1, Laktionov P.P.1, Kondakova I.V.3
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine
- Novosibirsk State University
- Tomsk Cancer Research Institute
- Novosibirsk Regional Oncological Dispensary
- Issue: Vol 11, No 3 (2017)
- Pages: 291-295
- Section: Article
- URL: https://journals.rcsi.science/1990-7508/article/view/197929
- DOI: https://doi.org/10.1134/S1990750817030106
- ID: 197929
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Abstract
A simple approach for isolation of exosomes from blood plasma samples has been proposed. Using this approach it is possible to obtain highly purified preparations of microvesicles no larger than 100 nm. The presence of different subpopulations of exosomes isolated by this method has been recognized in the blood plasma of healthy donors and cancer patients. Universal markers CD9, CD24, and CD81 are applicable for routine typing of exosomes isolated from blood plasma samples.
About the authors
S. N. Tamkovich
Institute of Chemical Biology and Fundamental Medicine; Novosibirsk State University
Author for correspondence.
Email: s.tamk@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090; Novosibirsk
N. V. Yunusova
Tomsk Cancer Research Institute
Email: s.tamk@niboch.nsc.ru
Russian Federation, Tomsk
M. N. Stakheeva
Tomsk Cancer Research Institute
Email: s.tamk@niboch.nsc.ru
Russian Federation, Tomsk
A. K. Somov
Institute of Chemical Biology and Fundamental Medicine
Email: s.tamk@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090
A. E. Frolova
Tomsk Cancer Research Institute
Email: s.tamk@niboch.nsc.ru
Russian Federation, Tomsk
N. A. Kiryushina
Novosibirsk Regional Oncological Dispensary
Email: s.tamk@niboch.nsc.ru
Russian Federation, Novosibirsk
S. G. Afanasyev
Tomsk Cancer Research Institute
Email: s.tamk@niboch.nsc.ru
Russian Federation, Tomsk
A. E. Grigor’eva
Institute of Chemical Biology and Fundamental Medicine
Email: s.tamk@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090
P. P. Laktionov
Institute of Chemical Biology and Fundamental Medicine
Email: s.tamk@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090
I. V. Kondakova
Tomsk Cancer Research Institute
Email: s.tamk@niboch.nsc.ru
Russian Federation, Tomsk
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