PIP1 aquaporins, sterols, and osmotic water permeability of plasma membranes from etiolated pea seedlings


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Plasma membrane isolated from microsomal membranes of pea seedling root and shoot cells by means of aqueous two-phase polymer system was separated by flotation in discontinuous OptiPrep gradient into “light” (≤1.146 g/cm3) and “heavy” (≥1.146 g/cm3) fractions. Osmotic water permeability of plasma membrane and its two fractions was investigated by inducing transmembrane osmotic gradient on the vesicle membrane and recording the kinetics of vesicle osmotic shrinkage by the stopped-flow method. Rate constants of osmotic shrinkage and coefficients of osmotic water permeability of the membranes were estimated on the basis of the kinetic curve approximation by exponential dependencies and using electron microscope data on vesicles sizes. In plasma membrane and its fractions the content of sterols and PIP1 aquaporins was determined. It was found that in “light” PM fractions from both roots and shoots the content of PIP1 aquaporins and sterols was higher and the osmotic water permeability coefficient was lower than in “heavy” fractions of plasma membrane. The results indicate that plasma membrane of roots and shoots is heterogeneous in osmotic water permeability. This heterogeneity may be related with the presence of microdomains with different content of aquaporins and sterols in the membrane.

Авторлар туралы

B. Belugin

Timiryazev Institute of Plant Physiology

Email: troms@ippras.ru
Ресей, Botanicheskaya ul. 35, Moscow, 127276

I. Zhestkova

Timiryazev Institute of Plant Physiology

Email: troms@ippras.ru
Ресей, Botanicheskaya ul. 35, Moscow, 127276

M. Piotrovskii

Timiryazev Institute of Plant Physiology

Email: troms@ippras.ru
Ресей, Botanicheskaya ul. 35, Moscow, 127276

N. Lapshin

Timiryazev Institute of Plant Physiology

Email: troms@ippras.ru
Ресей, Botanicheskaya ul. 35, Moscow, 127276

M. Trofimova

Timiryazev Institute of Plant Physiology

Хат алмасуға жауапты Автор.
Email: troms@ippras.ru
Ресей, Botanicheskaya ul. 35, Moscow, 127276


© Pleiades Publishing, Ltd., 2017

Осы сайт cookie-файлдарды пайдаланады

Біздің сайтты пайдалануды жалғастыра отырып, сіз сайттың дұрыс жұмыс істеуін қамтамасыз ететін cookie файлдарын өңдеуге келісім бересіз.< / br>< / br>cookie файлдары туралы< / a>