PIP1 aquaporins, sterols, and osmotic water permeability of plasma membranes from etiolated pea seedlings
- 作者: Belugin B.1, Zhestkova I.1, Piotrovskii M.1, Lapshin N.1, Trofimova M.1
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隶属关系:
- Timiryazev Institute of Plant Physiology
- 期: 卷 11, 编号 2 (2017)
- 页面: 168-176
- 栏目: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213248
- DOI: https://doi.org/10.1134/S1990747817020039
- ID: 213248
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详细
Plasma membrane isolated from microsomal membranes of pea seedling root and shoot cells by means of aqueous two-phase polymer system was separated by flotation in discontinuous OptiPrep gradient into “light” (≤1.146 g/cm3) and “heavy” (≥1.146 g/cm3) fractions. Osmotic water permeability of plasma membrane and its two fractions was investigated by inducing transmembrane osmotic gradient on the vesicle membrane and recording the kinetics of vesicle osmotic shrinkage by the stopped-flow method. Rate constants of osmotic shrinkage and coefficients of osmotic water permeability of the membranes were estimated on the basis of the kinetic curve approximation by exponential dependencies and using electron microscope data on vesicles sizes. In plasma membrane and its fractions the content of sterols and PIP1 aquaporins was determined. It was found that in “light” PM fractions from both roots and shoots the content of PIP1 aquaporins and sterols was higher and the osmotic water permeability coefficient was lower than in “heavy” fractions of plasma membrane. The results indicate that plasma membrane of roots and shoots is heterogeneous in osmotic water permeability. This heterogeneity may be related with the presence of microdomains with different content of aquaporins and sterols in the membrane.
作者简介
B. Belugin
Timiryazev Institute of Plant Physiology
Email: troms@ippras.ru
俄罗斯联邦, Botanicheskaya ul. 35, Moscow, 127276
I. Zhestkova
Timiryazev Institute of Plant Physiology
Email: troms@ippras.ru
俄罗斯联邦, Botanicheskaya ul. 35, Moscow, 127276
M. Piotrovskii
Timiryazev Institute of Plant Physiology
Email: troms@ippras.ru
俄罗斯联邦, Botanicheskaya ul. 35, Moscow, 127276
N. Lapshin
Timiryazev Institute of Plant Physiology
Email: troms@ippras.ru
俄罗斯联邦, Botanicheskaya ul. 35, Moscow, 127276
M. Trofimova
Timiryazev Institute of Plant Physiology
编辑信件的主要联系方式.
Email: troms@ippras.ru
俄罗斯联邦, Botanicheskaya ul. 35, Moscow, 127276