Email this article Applicability of TOF-SIMS for the assessment of lipid composition of cell membrane structures
- Authors: Gulin A.A.1,2, Pavlyukov M.S.3, Gusev S.A.4, Malakhova Y.N.5,6, Buzin A.I.7, Chvalun S.N.5,7, Aldarov K.G.4, Klinov D.V.4, Gularyan S.K.1, Nadtochenko V.A.1,2
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Affiliations:
- Semenov Institute of Chemical Physics
- Department of Chemistry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Federal Medical & Biological Agency SRI of Physical-Chemical Medicine
- National Research Centre “Kurchatov Institute”
- Institute of Fine Chemical Technology
- Enikolopov Institute of Synthetic Polymer Materials
- Issue: Vol 11, No 2 (2017)
- Pages: 144-150
- Section: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213245
- DOI: https://doi.org/10.1134/S1990747817020052
- ID: 213245
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Abstract
Langmuir–Blodgett lipid films and plasma membranes of glioma cells were analyzed using timeof-flight secondary ion mass spectrometry (TOF-SIMS). Bi3+ primary ion beam was determined to be most efficient in various experimental setups. TOF-SIMS was shown to be applicable for a quantitative analysis of model lipid structures, as well as plasma membranes of glioma cells U87MG in vitro. A combination of atomic-force microscopy and scanning electron microscopy yielded the depth resolution of ~10–20 nm for cell surface scanning by primary ion beam.
About the authors
A. A. Gulin
Semenov Institute of Chemical Physics; Department of Chemistry
Author for correspondence.
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Kosygina 4, Moscow, 119991; Leninskiye Gory 1, build. 3, Moscow, 119991
M. S. Pavlyukov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
S. A. Gusev
Federal Medical & Biological Agency SRI of Physical-Chemical Medicine
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119992
Yu. N. Malakhova
National Research Centre “Kurchatov Institute”; Institute of Fine Chemical Technology
Email: aleksandr.gulin@phystech.edu
Russian Federation, pl. Akademika Kurchatova 1, Moscow, 123182; pr. Vernadskogo 86, Moscow, 119571
A. I. Buzin
Enikolopov Institute of Synthetic Polymer Materials
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Profsoyuznaya 70, Moscow, 117393
S. N. Chvalun
National Research Centre “Kurchatov Institute”; Enikolopov Institute of Synthetic Polymer Materials
Email: aleksandr.gulin@phystech.edu
Russian Federation, pl. Akademika Kurchatova 1, Moscow, 123182; ul. Profsoyuznaya 70, Moscow, 117393
K. G. Aldarov
Federal Medical & Biological Agency SRI of Physical-Chemical Medicine
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119992
D. V. Klinov
Federal Medical & Biological Agency SRI of Physical-Chemical Medicine
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119992
S. K. Gularyan
Semenov Institute of Chemical Physics
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Kosygina 4, Moscow, 119991
V. A. Nadtochenko
Semenov Institute of Chemical Physics; Department of Chemistry
Email: aleksandr.gulin@phystech.edu
Russian Federation, ul. Kosygina 4, Moscow, 119991; Leninskiye Gory 1, build. 3, Moscow, 119991
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