电邮这篇文章 Differential expression of CD42b and CD9 proteins in platelets and extracellular membrane vesicles during platelet-concentrate storage
- 作者: Kishenko V.1, Kondratov K.1, Belyakova M.1, Mikhailovskii V.2, Sidorkevich S.1, Vavilova T.1, Fedorov A.1,3, Sirotkina O.1,4
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隶属关系:
- Almazov National Medical Research Centre
- Interdisciplinary Resource Center for Nanotechnology
- Department of Cytology and Histology
- Konstantinov Petersburg Nuclear Physics Institute
- 期: 卷 11, 编号 6 (2017)
- 页面: 440-446
- 栏目: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212493
- DOI: https://doi.org/10.1134/S1990519X17060050
- ID: 212493
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详细
Platelet concentrate is used to restore and maintain hemostasis in patients with a reduced number or activity of platelets. Platelet concentrate changes its properties when stored, which leads to a weakening of the therapeutic effect, as well as the occurrence of transfusion side effects. One of the processes that alter platelet concentrates during storage is the secretion of several types of membrane vesicles. Despite the fact that platelet-derived membrane vesicles affect homeostasis and transmit signals of intercellular communication, mechanisms of their formation, features of regulatory activity and molecular composition are still poorly understood. In this paper, the results of analysis of CD42b and CD9 membrane proteins expression in platelets and platelet-derived membrane vesicles during platelet-concentrate storage are presented. Populations of membrane vesicles of different sizes were isolated from the platelet concentrates and characterized. Aggregation and morphological alteration of platelets are observed during storage; the protein composition of platelets, as well as membrane vesicles, changes, and there is a significant increase in the levels of CD42b and CD9 proteins in fractions of membrane vesicles. The results obtained indicate that platelet concentrates contain different populations of membrane vesicles, the molecular composition of which varies during storage.
作者简介
V. Kishenko
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341
K. Kondratov
Almazov National Medical Research Centre
编辑信件的主要联系方式.
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341
M. Belyakova
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341
V. Mikhailovskii
Interdisciplinary Resource Center for Nanotechnology
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 199034
S. Sidorkevich
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341
T. Vavilova
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341
A. Fedorov
Almazov National Medical Research Centre; Department of Cytology and Histology
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341; St. Petersburg, 199034
O. Sirotkina
Almazov National Medical Research Centre; Konstantinov Petersburg Nuclear Physics Institute
Email: kondratovk.kirill@yandex.ru
俄罗斯联邦, St. Petersburg, 197341; Gatchina, Leningrad oblast, 188300
