Email this article Differential expression of CD42b and CD9 proteins in platelets and extracellular membrane vesicles during platelet-concentrate storage
- Authors: Kishenko V.V.1, Kondratov K.A.1, Belyakova M.V.1, Mikhailovskii V.Y.2, Sidorkevich S.V.1, Vavilova T.V.1, Fedorov A.V.1,3, Sirotkina O.V.1,4
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Affiliations:
- Almazov National Medical Research Centre
- Interdisciplinary Resource Center for Nanotechnology
- Department of Cytology and Histology
- Konstantinov Petersburg Nuclear Physics Institute
- Issue: Vol 11, No 6 (2017)
- Pages: 440-446
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212493
- DOI: https://doi.org/10.1134/S1990519X17060050
- ID: 212493
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Abstract
Platelet concentrate is used to restore and maintain hemostasis in patients with a reduced number or activity of platelets. Platelet concentrate changes its properties when stored, which leads to a weakening of the therapeutic effect, as well as the occurrence of transfusion side effects. One of the processes that alter platelet concentrates during storage is the secretion of several types of membrane vesicles. Despite the fact that platelet-derived membrane vesicles affect homeostasis and transmit signals of intercellular communication, mechanisms of their formation, features of regulatory activity and molecular composition are still poorly understood. In this paper, the results of analysis of CD42b and CD9 membrane proteins expression in platelets and platelet-derived membrane vesicles during platelet-concentrate storage are presented. Populations of membrane vesicles of different sizes were isolated from the platelet concentrates and characterized. Aggregation and morphological alteration of platelets are observed during storage; the protein composition of platelets, as well as membrane vesicles, changes, and there is a significant increase in the levels of CD42b and CD9 proteins in fractions of membrane vesicles. The results obtained indicate that platelet concentrates contain different populations of membrane vesicles, the molecular composition of which varies during storage.
About the authors
V. V. Kishenko
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341
K. A. Kondratov
Almazov National Medical Research Centre
Author for correspondence.
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341
M. V. Belyakova
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341
V. Yu. Mikhailovskii
Interdisciplinary Resource Center for Nanotechnology
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 199034
S. V. Sidorkevich
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341
T. V. Vavilova
Almazov National Medical Research Centre
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341
A. V. Fedorov
Almazov National Medical Research Centre; Department of Cytology and Histology
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341; St. Petersburg, 199034
O. V. Sirotkina
Almazov National Medical Research Centre; Konstantinov Petersburg Nuclear Physics Institute
Email: kondratovk.kirill@yandex.ru
Russian Federation, St. Petersburg, 197341; Gatchina, Leningrad oblast, 188300
