Optimal Methods of Cell Seeding and Cultivation on a Poly(L-lactide) Biodegradable Scaffold
- Авторы: Popov G.1, Kryukov A.2,3, Popryadukhin P.2,3, Naschekina Y.3,4, Ivankova E.2,3, Vavilov V.1, Yudin V.2,3, Smirnova N.2
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Учреждения:
- Pavlov First St. Petersburg State Medical University
- Institute of Macromolecular Compounds, Russian Academy of Sciences
- Peter the Great St. Petersburg State Polytechnical University
- Institute of Cytology of the Russian Academy of Sciences
- Выпуск: Том 12, № 5 (2018)
- Страницы: 359-366
- Раздел: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212740
- DOI: https://doi.org/10.1134/S1990519X1805005X
- ID: 212740
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Аннотация
In modern cardiovascular surgery, there remains a problem associated with a lack of venous material for bypass operations. Great interest has emerged in developing small-diameter vascular grafts ensuring stable patency. One possible solution is the creation of a tissue-engineered vascular implant (TEVI) based on a biodegradable polymer scaffold. Being structural and functional features of the scaffold, cell integration and cultivation on the scaffold are the key processes in the TEVI development. The aim of this research is to identify the optimal method of adipose-derived mesenchymal stem cell seeding and cultivation on the tubular biodegradable scaffold from poly(L-lactide). Results of static and dynamic cells cultivation on the scaffold after seeding with the developed filtration method were compared. The proposed method combining filtration seeding and dynamic cultivation has proved its higher efficiency and is suitable for further TEVI development.
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Об авторах
G. Popov
Pavlov First St. Petersburg State Medical University
Автор, ответственный за переписку.
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 197022
A. Kryukov
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great St. Petersburg State Polytechnical University
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 199004; St. Petersburg, 194064
P. Popryadukhin
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great St. Petersburg State Polytechnical University
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 199004; St. Petersburg, 194064
Yu. Naschekina
Peter the Great St. Petersburg State Polytechnical University; Institute of Cytology of the Russian Academy of Sciences
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 194064; St. Petersburg, 194064
E. Ivankova
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great St. Petersburg State Polytechnical University
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 199004; St. Petersburg, 194064
V. Vavilov
Pavlov First St. Petersburg State Medical University
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 197022
V. Yudin
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great St. Petersburg State Polytechnical University
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 199004; St. Petersburg, 194064
N. Smirnova
Institute of Macromolecular Compounds, Russian Academy of Sciences
Email: trek-4300@yandex.ru
Россия, St. Petersburg, 199004