The Effect of TCR-Activation and β-Estradiol on the Maturation and Differentiation of CD45RA+ T cells in vitro
- Autores: Khaziakhmatova O.1, Yurova K.1, Todosenko N.1, Litvinova L.1
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Afiliações:
- Basic Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University
- Edição: Volume 12, Nº 6 (2018)
- Páginas: 448-454
- Seção: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212785
- DOI: https://doi.org/10.1134/S1990519X18060032
- ID: 212785
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Resumo
Cellular and molecular aspects of maturation and differentiation of TCR-activated naive (CD3+CD45RA+CD62L+) T cells exposed to different concentrations of the female sex hormone β-estradiol in vitro were monitored. CD3+CD45RA+CD62L+ T cells were obtained from a fraction of blood mononuclear cells of healthy donors using immunomagnetic separation. The immunophenotype of T lymphocytes was analyzed by flow cytofluorometry. The polymerase chain reaction defined the level of U2af1l4 and Gfi1 gene expression in CD3+CD45RA+CD62L+ T cells. It was found that altered U2af1l4, Gfi1 gene expression produced by β-estradiol affected the maturation and differentiation of CD45RA+CD62L+ T lymphocytes. The cells underwent phenotypic conversion characterized by increased numbers of CD45RO+-T lymphocytes and reduced in content of T cells with molecules of activation and costimulation (CD127 and CD28) on the membrane surface.
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Sobre autores
O. Khaziakhmatova
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
Rússia, Kaliningrad, 236029
K. Yurova
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
Rússia, Kaliningrad, 236029
N. Todosenko
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
Rússia, Kaliningrad, 236029
L. Litvinova
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Autor responsável pela correspondência
Email: larisalitvinova@yandex.ru
Rússia, Kaliningrad, 236029