The Effect of TCR-Activation and β-Estradiol on the Maturation and Differentiation of CD45RA+ T cells in vitro
- 作者: Khaziakhmatova O.1, Yurova K.1, Todosenko N.1, Litvinova L.1
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隶属关系:
- Basic Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University
- 期: 卷 12, 编号 6 (2018)
- 页面: 448-454
- 栏目: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212785
- DOI: https://doi.org/10.1134/S1990519X18060032
- ID: 212785
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详细
Cellular and molecular aspects of maturation and differentiation of TCR-activated naive (CD3+CD45RA+CD62L+) T cells exposed to different concentrations of the female sex hormone β-estradiol in vitro were monitored. CD3+CD45RA+CD62L+ T cells were obtained from a fraction of blood mononuclear cells of healthy donors using immunomagnetic separation. The immunophenotype of T lymphocytes was analyzed by flow cytofluorometry. The polymerase chain reaction defined the level of U2af1l4 and Gfi1 gene expression in CD3+CD45RA+CD62L+ T cells. It was found that altered U2af1l4, Gfi1 gene expression produced by β-estradiol affected the maturation and differentiation of CD45RA+CD62L+ T lymphocytes. The cells underwent phenotypic conversion characterized by increased numbers of CD45RO+-T lymphocytes and reduced in content of T cells with molecules of activation and costimulation (CD127 and CD28) on the membrane surface.
作者简介
O. Khaziakhmatova
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
俄罗斯联邦, Kaliningrad, 236029
K. Yurova
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
俄罗斯联邦, Kaliningrad, 236029
N. Todosenko
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
Email: larisalitvinova@yandex.ru
俄罗斯联邦, Kaliningrad, 236029
L. Litvinova
Basic Laboratory of Immunology and Cell Biotechnologies,Immanuel Kant Baltic Federal University
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Email: larisalitvinova@yandex.ru
俄罗斯联邦, Kaliningrad, 236029