New data on structures formed by the FtsZ protein during the cell division process in Escherichia coli obtained using the localization microscopy method
- Authors: Vedyaykin A.D.1, Vishnyakov I.E.1,2, Polinovskaya V.S.1, Artamonova T.O.1, Khodorkovskii M.A.1, Sabantsev A.V.1
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Affiliations:
- Peter the Great St. Petersburg Polytechnic University
- Institute of Cytology RAS
- Issue: Vol 10, No 1 (2016)
- Pages: 76-83
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212038
- DOI: https://doi.org/10.1134/S1990519X16010120
- ID: 212038
Cite item
Abstract
The FtsZ protein, a bacterial tubulin homolog, is one of the key proteins in bacterial cell division, forming a contractile Z-ring in the middle of the dividing cell. In the present study, immunofluorescence staining, in combination with the localization microscopy method, was used for visualization of the structures formed by unlabelled FtsZ in Escherrichia coli cells. The techniques employed allowed reconstruction of the multistep mechanism of formation of FtsZ structures during the cytokinesis process. New data were obtained confirming the hypothesis that FtsZ is a helixlike structure that constricts during the division, producing constriction between the daughter cells.
About the authors
A. D. Vedyaykin
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Russian Federation, St. Petersburg, 195251
I. E. Vishnyakov
Peter the Great St. Petersburg Polytechnic University; Institute of Cytology RAS
Email: sabantsev.a.v@gmail.com
Russian Federation, St. Petersburg, 195251; Saint Petersburg, 194064
V. S. Polinovskaya
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Russian Federation, St. Petersburg, 195251
T. O. Artamonova
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Russian Federation, St. Petersburg, 195251
M. A. Khodorkovskii
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Russian Federation, St. Petersburg, 195251
A. V. Sabantsev
Peter the Great St. Petersburg Polytechnic University
Author for correspondence.
Email: sabantsev.a.v@gmail.com
Russian Federation, St. Petersburg, 195251