New data on structures formed by the FtsZ protein during the cell division process in Escherichia coli obtained using the localization microscopy method
- Autores: Vedyaykin A.1, Vishnyakov I.1,2, Polinovskaya V.1, Artamonova T.1, Khodorkovskii M.1, Sabantsev A.1
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Afiliações:
- Peter the Great St. Petersburg Polytechnic University
- Institute of Cytology RAS
- Edição: Volume 10, Nº 1 (2016)
- Páginas: 76-83
- Seção: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212038
- DOI: https://doi.org/10.1134/S1990519X16010120
- ID: 212038
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Resumo
The FtsZ protein, a bacterial tubulin homolog, is one of the key proteins in bacterial cell division, forming a contractile Z-ring in the middle of the dividing cell. In the present study, immunofluorescence staining, in combination with the localization microscopy method, was used for visualization of the structures formed by unlabelled FtsZ in Escherrichia coli cells. The techniques employed allowed reconstruction of the multistep mechanism of formation of FtsZ structures during the cytokinesis process. New data were obtained confirming the hypothesis that FtsZ is a helixlike structure that constricts during the division, producing constriction between the daughter cells.
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Sobre autores
A. Vedyaykin
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Rússia, St. Petersburg, 195251
I. Vishnyakov
Peter the Great St. Petersburg Polytechnic University; Institute of Cytology RAS
Email: sabantsev.a.v@gmail.com
Rússia, St. Petersburg, 195251; Saint Petersburg, 194064
V. Polinovskaya
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Rússia, St. Petersburg, 195251
T. Artamonova
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Rússia, St. Petersburg, 195251
M. Khodorkovskii
Peter the Great St. Petersburg Polytechnic University
Email: sabantsev.a.v@gmail.com
Rússia, St. Petersburg, 195251
A. Sabantsev
Peter the Great St. Petersburg Polytechnic University
Autor responsável pela correspondência
Email: sabantsev.a.v@gmail.com
Rússia, St. Petersburg, 195251