Email this article Temporal Parameters of p53-GFP Protein Transfer via Exosomes in Cocultured HEK293 and GFP-HEK293 Cells
- Authors: Pantina R.A.1, Varfolomeeva E.Y.1, Burdakov V.S.1,2, Landa S.B.1, Bayramukov V.Y.1, Kovalev R.A.1, Filatov M.V.1,3
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Affiliations:
- St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute
- Peter the Great St. Petersburg Polytechnic University
- St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation
- Issue: Vol 13, No 3 (2019)
- Pages: 188-197
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212932
- DOI: https://doi.org/10.1134/S1990519X1903009X
- ID: 212932
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Abstract
In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while the original HEK293 cell line was used as a recipient. Our results provide evidence that the transfer of the GFP protein from cell to cell is carried out via exosomes. It has been shown that the accumulation of this protein by the recipient cells in cocultures of these cells takes a prolonged time. The temporal parameters of this transfer differ between the cells within the same population.
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About the authors
R. A. Pantina
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300
E. Yu. Varfolomeeva
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300
V. S. Burdakov
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute; Peter the Great St. Petersburg Polytechnic University
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300; St. Petersburg, 194064
S. B. Landa
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300
V. Yu. Bayramukov
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300
R. A. Kovalev
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300
M. V. Filatov
St. Petersburg Nuclear Physics Institute Named after B.P. Konstantinov of National Research Centre Kurchatov Institute; St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation
Author for correspondence.
Email: fil_53@mail.ru
Russian Federation, Gatchina, 188300; St. Petersburg, 191036
