Extracellular pH Regulates the Entry of Calcium into Jurkat T-cells
- Authors: Cherezova A.L.1, Negulyaev J.A.1, Zenin V.V.1, Semenova S.B.1
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Affiliations:
- Institute of Cytology of the Russian Academy of Sciences
- Issue: Vol 12, No 1 (2018)
- Pages: 41-47
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212554
- DOI: https://doi.org/10.1134/S1990519X18010042
- ID: 212554
Cite item
Abstract
The entry of calcium into blood cells is a key event in the launching of multiple transcription programs and in the development of immune response, proliferation, growth and cell differentiation. The TRPchannel superfamily serves as the main conductor of calcium ions in blood cells, most of the regulatory elements of which remain unknown. In the current work carried out on Jurkat cells using the patch-clamp method, we showed that the activity of TRPV5 and TRPV6 calcium channels (transient receptor potential vanilloid) of the TRP superfamily depends on the pH of the environment. Results of measurements of the intracellular Ca2+ level using a fluorescent Ca2+-specific Fluo-4 probe demonstrated that the entry of Ca2+ into Jurkat cells is also regulated by extracellular pH. The obtained data indicate that changes in the pH of the environment affecting the activity of the TRPV5 and TRPV6 calcium channels can regulate the entry and level of intracellular Ca2+ in Jurkat cells. It is suggested that such a mechanism for responding to changes in the acid–base balance is necessary for the cells of the immune system to trigger protective reactions, including the areas of development of various inflammations.
Keywords
About the authors
A. L. Cherezova
Institute of Cytology of the Russian Academy of Sciences
Email: svsem@incras.ru
Russian Federation, St. Petersburg, 194064
Ju. A. Negulyaev
Institute of Cytology of the Russian Academy of Sciences
Email: svsem@incras.ru
Russian Federation, St. Petersburg, 194064
V. V. Zenin
Institute of Cytology of the Russian Academy of Sciences
Email: svsem@incras.ru
Russian Federation, St. Petersburg, 194064
S. B. Semenova
Institute of Cytology of the Russian Academy of Sciences
Author for correspondence.
Email: svsem@incras.ru
Russian Federation, St. Petersburg, 194064