Extracellular pH Regulates the Entry of Calcium into Jurkat T-cells
- 作者: Cherezova A.1, Negulyaev J.1, Zenin V.1, Semenova S.1
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隶属关系:
- Institute of Cytology of the Russian Academy of Sciences
- 期: 卷 12, 编号 1 (2018)
- 页面: 41-47
- 栏目: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212554
- DOI: https://doi.org/10.1134/S1990519X18010042
- ID: 212554
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详细
The entry of calcium into blood cells is a key event in the launching of multiple transcription programs and in the development of immune response, proliferation, growth and cell differentiation. The TRPchannel superfamily serves as the main conductor of calcium ions in blood cells, most of the regulatory elements of which remain unknown. In the current work carried out on Jurkat cells using the patch-clamp method, we showed that the activity of TRPV5 and TRPV6 calcium channels (transient receptor potential vanilloid) of the TRP superfamily depends on the pH of the environment. Results of measurements of the intracellular Ca2+ level using a fluorescent Ca2+-specific Fluo-4 probe demonstrated that the entry of Ca2+ into Jurkat cells is also regulated by extracellular pH. The obtained data indicate that changes in the pH of the environment affecting the activity of the TRPV5 and TRPV6 calcium channels can regulate the entry and level of intracellular Ca2+ in Jurkat cells. It is suggested that such a mechanism for responding to changes in the acid–base balance is necessary for the cells of the immune system to trigger protective reactions, including the areas of development of various inflammations.
作者简介
A. Cherezova
Institute of Cytology of the Russian Academy of Sciences
Email: svsem@incras.ru
俄罗斯联邦, St. Petersburg, 194064
Ju. Negulyaev
Institute of Cytology of the Russian Academy of Sciences
Email: svsem@incras.ru
俄罗斯联邦, St. Petersburg, 194064
V. Zenin
Institute of Cytology of the Russian Academy of Sciences
Email: svsem@incras.ru
俄罗斯联邦, St. Petersburg, 194064
S. Semenova
Institute of Cytology of the Russian Academy of Sciences
编辑信件的主要联系方式.
Email: svsem@incras.ru
俄罗斯联邦, St. Petersburg, 194064