Evaluation of the vasculogenic potential of hydrogels based on modified fibrin
- Authors: Shpichka A.I.1, Koroleva A.V.2, Deiwick A.2, Timashev P.S.3,4, Semenova E.F.1, Moiseeva I.Y.1, Konoplyannikov M.A.5, Chichkov B.N.2
-
Affiliations:
- Department of General and Clinical Pharmacology
- Laser Zentrum Hannover e.V.
- Institute of Photonic Technologies, Research center “Crystallography and Photonics”
- Sechenov First Moscow State Medical University
- Federal Scientific Clinical Center of Specialized Types of Medical Aid and Medical Technologies of Federal Medical Biological Agency
- Issue: Vol 11, No 1 (2017)
- Pages: 81-87
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212282
- DOI: https://doi.org/10.1134/S1990519X17010126
- ID: 212282
Cite item
Abstract
The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.
About the authors
A. I. Shpichka
Department of General and Clinical Pharmacology
Author for correspondence.
Email: ana-shpichka@yandex.ru
Russian Federation, Penza, 440026
A. V. Koroleva
Laser Zentrum Hannover e.V.
Email: ana-shpichka@yandex.ru
Germany, Hannover, 30419
A. Deiwick
Laser Zentrum Hannover e.V.
Email: ana-shpichka@yandex.ru
Germany, Hannover, 30419
P. S. Timashev
Institute of Photonic Technologies, Research center “Crystallography and Photonics”; Sechenov First Moscow State Medical University
Email: ana-shpichka@yandex.ru
Russian Federation, Troitsk, Moscow, 142092; Moscow, 119991
E. F. Semenova
Department of General and Clinical Pharmacology
Email: ana-shpichka@yandex.ru
Russian Federation, Penza, 440026
I. Ya. Moiseeva
Department of General and Clinical Pharmacology
Email: ana-shpichka@yandex.ru
Russian Federation, Penza, 440026
M. A. Konoplyannikov
Federal Scientific Clinical Center of Specialized Types of Medical Aid and Medical Technologies of Federal Medical Biological Agency
Email: ana-shpichka@yandex.ru
Russian Federation, Moscow, 115682
B. N. Chichkov
Laser Zentrum Hannover e.V.
Email: ana-shpichka@yandex.ru
Germany, Hannover, 30419