Evaluation of the vasculogenic potential of hydrogels based on modified fibrin
- Авторы: Shpichka A.1, Koroleva A.2, Deiwick A.2, Timashev P.3,4, Semenova E.1, Moiseeva I.1, Konoplyannikov M.5, Chichkov B.2
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Учреждения:
- Department of General and Clinical Pharmacology
- Laser Zentrum Hannover e.V.
- Institute of Photonic Technologies, Research center “Crystallography and Photonics”
- Sechenov First Moscow State Medical University
- Federal Scientific Clinical Center of Specialized Types of Medical Aid and Medical Technologies of Federal Medical Biological Agency
- Выпуск: Том 11, № 1 (2017)
- Страницы: 81-87
- Раздел: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212282
- DOI: https://doi.org/10.1134/S1990519X17010126
- ID: 212282
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Аннотация
The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.
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Об авторах
A. Shpichka
Department of General and Clinical Pharmacology
Автор, ответственный за переписку.
Email: ana-shpichka@yandex.ru
Россия, Penza, 440026
A. Koroleva
Laser Zentrum Hannover e.V.
Email: ana-shpichka@yandex.ru
Германия, Hannover, 30419
A. Deiwick
Laser Zentrum Hannover e.V.
Email: ana-shpichka@yandex.ru
Германия, Hannover, 30419
P. Timashev
Institute of Photonic Technologies, Research center “Crystallography and Photonics”; Sechenov First Moscow State Medical University
Email: ana-shpichka@yandex.ru
Россия, Troitsk, Moscow, 142092; Moscow, 119991
E. Semenova
Department of General and Clinical Pharmacology
Email: ana-shpichka@yandex.ru
Россия, Penza, 440026
I. Moiseeva
Department of General and Clinical Pharmacology
Email: ana-shpichka@yandex.ru
Россия, Penza, 440026
M. Konoplyannikov
Federal Scientific Clinical Center of Specialized Types of Medical Aid and Medical Technologies of Federal Medical Biological Agency
Email: ana-shpichka@yandex.ru
Россия, Moscow, 115682
B. Chichkov
Laser Zentrum Hannover e.V.
Email: ana-shpichka@yandex.ru
Германия, Hannover, 30419