Ex vivo study of the kinetics of ovarian tissue optical properties under the influence of 40%-glucose

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Abstract

Background and Objectives: The reproductive system of women is a subject of diverse multidisciplinary research worldwide. These are oncological diseases, infertility of an unspecified nature, ovarian cryopreservation to preserve fertility, improvement of early diagnosis of diseases, etc. Elucidation of the mechanisms of diffusion of tissue water and hyperosmotic agents in luteal phase ovarian tissues controlled by аngiogenic growth factors can bring us closer to understanding biophysical processes in general. Materials and Methods. The work used spectroscopy of diffuse reflection, a free diffusion model and the modified Bouguer–Lambert–Beer law. Results. The diffusion coefficient of 40% glucose/tissue water intothe ovariantissue ofthe luteal phase D andthe diffusion time τ have been determined, which was D = (8.6 ± 1.4)·107 cm2 /s and τ = 50.4 ± 1.7 min with a samplethickness of(0.8 ± 0.1)mm. The efficiency of optical clearing of cat ovariantissues with 40% glucose immersion has been determined. Conclusions. Studies have shown that 40%-glucose is an effective optical clearing agent for topical use in differentiating normal and pathological ovarian tissues and in clinical applications.

About the authors

Alexey Andreevich Selifonov

Education and Research Institute of Nanostructures and Biosystems, Saratov State University

Email: skripalav@info.sgu.ru
83 Astrakhanskaya St., Saratov 410012, Russia

Andrey Sergeevich Rykhlov

Clinic “Veterinary Hospital”, Saratov State University of Genetics, Biotechnology and Engineering named after N. I. Vavilov

Email: skripalav@info.sgu.ru
220, Bolshaya Sadovaya St., Saratov 410012, Russia

Valery Viсtorovich Tuchin

Science Medical Center, Saratov State University

Author for correspondence.
Email: skripalav@info.sgu.ru
83 Astrakhanskaya St., Saratov 410012, Russia

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