Diversity of rhizosphere bacteria of halophytes of the coastal strip of lake Elton

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Abstract

Halophilic microorganisms attract the attention of researchers due to their ability, acquired during adaptation to existence in extreme conditions, to synthesize biopolymers with unique properties that are potentially in demand in biotechnology. Bacteria inhabiting the rhizosphere of plants growing in soils with an increased level of mineralization often demonstrate growth-stimulating activity. The aim of the study was to analyze the taxonomic diversity of rhizosphere bacteria of the predominant halophytes of the coastal strip of the salty endorheic sedimentary lake Elton (Volgograd region). Enrichment cultures of microorganisms were obtained from rhizosphere samples of plants of the Chenopodiaceae family, with subsequent isolation of bacterial DNA, amplifi cation and sequencing of nucleotide sequences of variable fragments V3-V4 of the 16S ribosomal RNA gene. The analysis of the rhizosphere microbiome of Climacoptera lachnophylla (Iljin) Botsch., Salsola soda L., Anabasis salsa (C. A. Mey.) Benth. ex Volkens demonstrated a higher species diversity compared to the microbiomes of the soil and salt samples from the lake’s coastal zone. The dominance of the Proteobacteria and Firmicutes phyla was noted in all analyzed samples with diff erent ratios of their representatives. The rhizosphere microbiota of the studied plants was represented mainly by known species of the Halomonadaceae and Bacillaceae families, which, according to literature data, quite often have the ability to produce such metabolites regulating plant development as indole-3-acetic acid and siderophores.

About the authors

Nikita Nikolaevich Lipatov

Saratov State University; Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)

ORCID iD: 0009-0005-5667-3604
83, Astrakhanskaya str., Saratov, 410012, Russia

Natalya S. Velichko

Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)

ORCID iD: 0000-0001-9734-3947
Scopus Author ID: 57204060745
ResearcherId: I-9974-2018
410049, Russia, Saratov, Entuziastov Avenue, 13

Elena N. Sigida

Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)

410049, Russia, Saratov, Entuziastov Avenue, 13

Yulia P. Fedonenko

Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)

410049, Russia, Saratov, Entuziastov Avenue, 13

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