Gender analysis of the microbiota of periodontal pockets in patients with chronical generalized periodontitis

Qualitative assessment of bacterial colonization and mRNA of human protective factors in periodontal swabs by using dPCR revealed principal differences between males and females. We demonstrated that concentration of transcripts encoding tumor necrosis factor (TNF-alpha), matrix metalloprotease 8 and 9 (MMP8 and MMP9) changes synchronously at periodontium of healthy men, but not in women and men with the chronic periodontitis. This observation correlates with a new fact of the most tight statistical association of the periodontal hyper-colonization with Porhyromonas gingivalis colonization with the severity of the chronic periodontitis in women but not in men. In contrast, Tanerella forsythensis or its complex with Treponema denticola are the only pathogen whose prevalence is statistically associated with the chronic periodontitis in men. Despite of similar extent ofperiodontium colonization with the pathogens in the males and the females, the women are substantially more prone to onset of the chronic periodontitis at the same level of the periodontal pathogen colonization. On the other hand, the males are inclined to a multiple infection with several principal periodontal pathogens, whereas the females more often are subjected to a mono-infection. Taken together, these facts allow a hypothesis that mechanisms of periodontium protection are different in males and females. The periodontal pathogens quantified by dPCR provide a better predictor of the periodontitis in the females then in the males.

Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Treponema denticola, periodontium, chronic periodontitis, PCR, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, treponema denticola