Efficiency of antiseptic agents for treatment of autogenous dentinal blocks

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Abstract

BACKGROUND: Autogenous material is currently one of the most common and effective materials used for bone grafting. In recent years, materials based on extracted teeth have attracted attention. When analyzing modern articles, all authors agree that before using autogenous dentinal material, it is necessary to carry out antiseptic treatment to prevent possible complications.

AIM: A comparison was made of various antiseptic agents for the treatment of autogenous dentinal blocks with subsequent osteoplastic operations in the pre-implantation period.

MATERIALS AND METHODS: At the Department of Maxillofacial and Plastic Surgery of the Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, 43 teeth were removed for orthodontic indications, the teeth were processed mechanically and fragmented into 125 autogenous dentinal blocks. Then they were treated with antiseptic agents (0.01% Miramistin® solution, 95% ethyl alcohol solution, 0.05% and 2% chlorhexidine solution) for 15 min and were sent to the Department of Microbiology, Virology, Immunology of the Moscow State University of Medicine and Dentistry named after A.I. Evdokimov for the isolation of microbial cultures.

RESULTS: The use of antiseptic agents showed a significant reduction in microbial contamination compared with the control group, which did not undergo antiseptic treatment, which indicates the effectiveness of the treatment of extracted teeth before using it as an osteoplastic material for bone grafting.

CONCLUSION: Autogenous dentinal blocks must be processed before being used as an osteoplastic material.

About the authors

Thanh H. Le

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

Author for correspondence.
Email: dr.thanhhieu@mail.ru
ORCID iD: 0009-0004-2061-1260

Postgraduate Student

Russian Federation, Moscow

Alexey Yu. Drobyshev

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

Email: dr.drobyshev@gmail.com
ORCID iD: 0000-0002-1710-6923
SPIN-code: 6683-8226

MD, Dr. Sci. (Med.), Professor

Russian Federation, Moscow

Nikolay A. Redko

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

Email: dr.redko@mail.ru
ORCID iD: 0000-0001-7807-9351
SPIN-code: 6189-4835

MD, Cand. Sci. (Med.), Assistant Lecturer

Russian Federation, Moscow

Viktor N. Tsarev

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

Email: nikola777@rambler.ru
ORCID iD: 0000-0002-3311-0367
SPIN-code: 8180-4941

MD, Dr. Sci. (Med.), Professor

Russian Federation, Moscow

Mikhail S. Podporin

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

Email: podporin.mikhail@yandex.ru
ORCID iD: 0000-0001-6785-0016
SPIN-code: 1937-4996

MD, Cand. Sci. (Med.), Research Associate

Russian Federation, Moscow

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Supplementary files

Supplementary Files
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2. Fig. 1. Distribution of samples of autogenous dentinal blocks into groups depending on the type of treatment.

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3. Fig. 2. Transport Swabs w/ Dey-Engley Neutralizing Broth (HiMedia, India).

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4. Fig. 3. Autogenous dentinal block placed in Transport Swabs w/ Dey-Engley Neutralizing Broth (HiMedia, India).

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5. Fig. 4. Microbial population of autogenous dentinal block in the control group (without treatment).

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6. Fig. 5. Microbial population of autogenous dentinal block in groups treated with 0.01% Miramistin solution and 95% ethyl alcohol solution.

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7. Fig. 6. Microbial population of autogenous dentinal blocks treated with 0.05% and 2% chlorhexidine solution.

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8. Fig. 7. Percentage analysis of the inoculation of representatives of microorganisms isolated from autogenous dentinal blocks in patients with various types of antiseptic treatment (with n strain=25, p <0.05).

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