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The effectiveness of autologous mesenchymal stem cells in the treatment of liver cirrhosis and the method of their visualization in the patient’s body
- Authors: Kotkas I.E.1, Enukashvili N.I.1,2, Asadulayev S.M.1, Chubar’ A.V.2
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Affiliations:
- North-Western State Medical University named after I.I. Mechnikov
- Institute of Cytology of the Russian Academy of Sciences
- Issue: Vol 22, No 3 (2020)
- Pages: 35-40
- Section: Clinical trials
- URL: https://journals.rcsi.science/1682-7392/article/view/50528
- DOI: https://doi.org/10.17816/brmma50528
- ID: 50528
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Abstract
Abstract. The effectiveness of the influence of autologous mesenchymal stem cells on the function of liver tissue in liver cirrhosis of alimentary etiology is considered, as well as the possibility of visualization of the introduced cell structures with subsequent cytological confirmation. To be able to track autologous mesenchymal stem cells introduced in the patient’s body, they were labeled with iron oxide nanoparticles. Visualization of the introduced cell structures was performed using magnetic resonance imaging and ultrasound examination of the liver. 6 months after the cell therapy, clinical and biochemical blood tests, lidocaine test results, elastography indicators were evaluated, and the dynamics of hepatic encephalopathy was evaluated. The effectiveness of using autologous mesenchymal stem cells was evidenced by the improvement of the above indicators. Also, 6 months after treatment, a liver tissue biopsy was performed from the sites of fixation of previously introduced cell structures. Histological examination revealed the largest number of labeled cells in the liver micro-nodes, as well as at the borders of micro-nodes and fibrous septa. The use of cell therapy in a patient suffering from liver cirrhosis of alimentary etiology helped to improve the indicators of laboratory and instrumental research methods. No complications were detected during the procedure.
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##article.viewOnOriginalSite##About the authors
I. E. Kotkas
North-Western State Medical University named after I.I. Mechnikov
Author for correspondence.
Email: inna.kotkas@yandex.ru
Russian Federation, Saint Petersburg
N. I. Enukashvili
North-Western State Medical University named after I.I. Mechnikov; Institute of Cytology of the Russian Academy of Sciences
Email: inna.kotkas@yandex.ru
Russian Federation, Saint Petersburg; Saint Petersburg
Sh. M. Asadulayev
North-Western State Medical University named after I.I. Mechnikov
Email: inna.kotkas@yandex.ru
Russian Federation, Saint Petersburg
A. V. Chubar’
Institute of Cytology of the Russian Academy of Sciences
Email: inna.kotkas@yandex.ru
Russian Federation, Saint Petersburg
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