Email this article 
PROTECTIVE EFFECT OF AMINO ACIDS IN THE ORGANOTYPIC CULTURE OF THE ECTODERMAL GENESIS TISSUES AT THE CYCLOPHOSPHANE PRESENCE
- Authors: Chalisova NI1,2, Korovin AE1
-
Affiliations:
- S. M. Kirov Military Medical Academy the Russian Defense Ministry, Saint Petersburg, Russia
- I. P. Pavlov Institute of physiology, Saint Petersburg
- Issue: Vol 19, No 4 (2017)
- Pages: 47-52
- Section: Articles
- URL: https://journals.rcsi.science/1682-7392/article/view/12431
- DOI: https://doi.org/10.17816/brmma12431
- ID: 12431
Cite item
Full Text
Abstract
The effect of the 20 coded amino acids was investigated on the development of the processes of the proliferation in the organotypic tissue culture of rat skin and brain cortex. Some amino acids at 0.05 ng/ml concentration stimulated the cellular proliferation in the growth zone of explants. The other inhibited it. The combination of the stimulating and inhibiting amino acids - Leucine with Proline or Tyrosine-, lead to the proliferation stimulation by 24-32%. The mustard-like agent cyclophosphane at 1 mg/ml concentration inhibited the cellular proliferation. However, the delay of this inhibiting effect of cyclophosphane was observed by the using of combination of amino acids with cyclophosphane. Thus, the amino acids can be protectors of the cellular proliferation by the toxic effect of the cyclophosphane on the tissues of the ectodermal genesis. This effect can be used for the treatment of the mustard injury of skin, brain cortex and for the delay of the adverse effect of cytostatic in oncology
Full Text
##article.viewOnOriginalSite##About the authors
N I Chalisova
S. M. Kirov Military Medical Academy the Russian Defense Ministry, Saint Petersburg, Russia; I. P. Pavlov Institute of physiology, Saint Petersburg
A E Korovin
S. M. Kirov Military Medical Academy the Russian Defense Ministry, Saint Petersburg, Russia
References
- Хавинсон В. Х., Чалисова Н. И., Линькова Н. С., Халимов Р. И., Ничик Т. Е. Зависимость тканеспецифического действия пептидов от количества аминокислот, входящих в их состав. Фундаментальные исследования. 2015; 2 (3): 497-503
- Чалисова Н. И., Концевая Е. А., Войцеховская М. А. Регуляторное влияние кодируемых аминокислот на основные клеточные процессы у молодых и старых животных. Успехи геронтологии. 2011; 24 (2): 189-97
- Chalisova N. I., Zakutzkii A. Eff ect of amino acids on cell proliferation and P53 expression in neonatal rats. Cell Biol. Int. 2008; 32 (2): 1574-7.
- Чалисова Н. И., Моралев С. Н. Статистический анализ данных о специфичности действия олигопептидов и аминокислот на ткани с различным генезом и функцией. Журнал эволюционной биохимии и физиологии. 2011; 47 (2): 519-21
- Kimball S. R., Jeff erson L. S. New functions for amino acids: eff ects on gene transcription and translation. Am. J. Clin. Nutr. 2006; 83 (2): 500-7.
- Neame K. D. Eff ect of neutral alpha- and omega-amino acids and basic alpha-amino acids on uptake of L-histidine by intestinal mucosa, testis, spleen and kidney in vitro: a comparison with eff ect in brain. J. Physiol. 1966; 185 (3): 627-45.
- Arai T., Hiromatsu K., Nishimura H., Kimura Y., Kobayashi N., Ishida H., Nimura Y., Yoshikai Y. Endogenous interleukin 10 prevents apoptosis in macrophages during Salmonella infection. Biochem. Biophys. Res. Commun. 1995; 213 (2): 600-7.
- Booth P. J., Humpherson P. G., Watson T. J., Leese H. J. Amino acid depletion and appearance during porcine preimplantation embryo development in vitro. Reproduction. 2005; 130 (5): 655-68.
- Fratelli M., Gagliardini V., Galli G., Gnocchi P., Ghiara P., Ghezzi P. Autocrine interleukin-1 beta regulates both proliferation and apoptosis in EL4-6.1 thymoma cells. Blood. 1995; 85 (12): 3532-7.
- Oehler R., Roth E. Regulative capacity of glutamine. Curr. Opin. Clin. Nutr. Metab. Care. 2003; 6 (3): 277-82.
- Cid C., Alvarez-Cermeno J. C., Regidor I., Plaza J., Salinas M., Alcázar A. Low concentrations of glutamate induce apoptosis in cultured neurons: implications for amyotrophic lateral sclerosis. J. Neurol. Sci. 2003; 206 (1): 91-5.
- Chen R. W., Qin Z. H., Ren M., Li M. Regulation of c-Jun N-terminal kinase, p38 kinase and AP-1 DNA binding in cultured brain neurons: roles in glutamate excitotoxicity and lithium neuroprotection. J. Neurochem. 2003; 84 (3): 566-75.
- Trulsson L., Sandström P., Sundqvist T., Smeds S., Gasslander T., Svanvik J. The Infl uence of a load of L-arginine on serum amino acids and pancreatic apoptosis/proliferation and ATP levels in the rat. Pancreas. 2004; 29 (4): 113-20.
- Kimura M., Ogihara M. Eff ects of branched-chain amino acids on DNA synthesis and proliferation in primary cultures of adult rat hepatocytes. Eur. J. Pharmacol. 2005; 510 (3): 167-180.
- Proud C. G. Amino acids and mTOR signalling in anabolic function. Biochem. Soc. Trans. 2005; 35 (5): 1187-90.
- Вахитов Т. Я., Чалисова Н. И., Салль Т. С., Концевая Е. А., Козина Л. С., Полевая Е. А., Заломаева Е. С. Влияние генетически кодируемых аминокислот и их предшественников карбоновых кислот на клеточную пролиферацию в органотипической культуре селезенки у молодых и старых крыс. Успехи геронтологии. 2017; 30 (1): 39-42
- Чалисова Н. И., Закуцкий А. Н., Анискина А. В., Ноздрачев А. Д. Влияние аргинина и его метаболитов на миокард крыс в органотипической культуре ткани. Доклады РАН. 2007;415 (2): 273-6).
- Чалисова Н.И., Пеннияйнен В.А., Ноздрачев А.Д. Регулирующее действие аминокислот в органотипической культуре лимфоидных тканей с различной степенью зрелости. Доклады РАН. 2003; 389 (2): 117-9).
Supplementary files
