Possibilities of biological control of extracorporeal photochemotherapy


Cite item

Full Text

Abstract

The results of the determination of levels of apoptosis in vitro in lymphocytes during the procedures of extracorporeal photochemotherapy (extracorporeal photopheresis) using flow cytometry are described. It was found that carrying out extracorporeal photopheresis does not have a significant effect on the viability of cells immediately after the procedures. Thus, the relative content of living cells in the samples after isolation of the mononuclear fraction of peripheral blood did not differ from both samples prepared for photopheresis and samples after this procedure. It should be noted that carrying out extracorporeal photopheresis does not lead to rapid cell death. At the same time, the level of living lymphocytes at the beginning of the experiment averaged about 90%, while the protocol used to extract the mononuclear fraction of peripheral blood cells and further manipulations with them in vitro allowed increasing the percentage of living cells in the samples to90% or more. An increase in the level of cells in the early stages of apoptosis occurs already in the first day after the beginning of the experiment, which is confirmed by the data of other researchers indicating that there are significant differences in the viability of cells with an initial point in the interval of 20-24 h in vitro incubation. The launch of the processes of programmed cell death in the case of own experiments was not related to the preparation of samples for extracorporeal photopheresis (as evidenced by the absence of significant differences between freshly isolated lymphocytes and samples prepared for the procedure), but with the procedure of photopheresis itself.

About the authors

A S Manuilov

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

I V Kudryavtsev

Институт экспериментальной медицины; Национальный медицинский исследовательский центр онкологии им. Н.Н. Петрова

Санкт-Петербург

M K Serebriakova

Институт экспериментальной медицины

Санкт-Петербург

A S Trulev

Институт экспериментальной медицины

Санкт-Петербург

S N Bardakov

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

A V Apchel

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

A N Belskih

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

M V Zakharov

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

V V Tishko

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

A A Sokolov

Военно-медицинская академия им. С.М. Кирова

Email: vmeda-nio@mil.ru
Санкт-Петербург

I A Vasylieva

Первый государственный медицинский университет им. академика И.П. Павлова

Санкт-Петербург

References

  1. Соколов, А.А. Эфферентные методы интенсивной терапии аутоиммунных и метаболических заболеваний (эксперимен- тально-клиническое исследование): дис. … д-ра мед. наук / А.А. Соколов. - СПб.: МАПО, 2007. - 505 с.
  2. Черешнев, В.А. Фармакологическое регулирование програм- мированной гибели клеток / В.А. Черешнев, В. Н. Цыган, М.М. Одинак. - СПб.: Наука, 2011. - 255 с.
  3. Barr, M. Photopheresis for the prevention of rejection in cardiac transplantation. Photopheresis Transplantation Study Group / M. Barr [et al.] // N. Engl. J. Med. - 1998. - Vol. 339. - P. 1744-1751.
  4. Bisaccia, E. Extracorporeal photochemotherapy for the treatment of refractory Crohn’s disease / E. Bisaccia [et al.] // Transfus. Apher. 2007. - Vol. 37 - P. 171-174
  5. Capuano, M. Current clinical applications of extracorporeal photochemotherapy / M. Capuano [et al.] // Therapeutic Apheresis and Dialysis - 2015. - Vol. 19, № 2. - P. 103-110.
  6. Cavaletti, G. Extracorporeal photochemotherapy: a safety and tolerability pilot study with preliminary efficacy results in refractory relapsing-remitting multiple sclerosis / G. Cavaletti [et al.] // Neurol. 2006. - Vol. 27. - P. 24-32.
  7. Daniele, N. Evaluation of cell death after treatment with extracorporeal photopheresis / N. Daniele [et al.] // Transfus. Apher. - 2012. - Vol. 46. - P. 53-57.
  8. Edelson, R, Treatment of cutaneous T-cell lymphoma by extracorporeal photochemotherapy. Preliminary results / R. Edelson // N. Engl. J. Med. - 1987. - Vol. 316. - P. 297-303.
  9. Goussetis, E. Update on the mechanism of action and on clinical efficacy of extracorporeal photopheresis in the treatment of acute and chronic graft versus host disease in children / E. Goussetis [et al.] // Transfus. Apher. 2012. - Vol. 46. - P. 203-209.
  10. Hannani, D. Extracorporeal Photopheresis: Tolerogenic or Immunogenic Cell Death? Beyond Current Dogma / D. Hannani // Front Immunol. 2015. - Vol. 7. - P. 346-349.
  11. Hart, J. Extracorporeal photopheresis in the treatment of graft- versus-host disease: evidence and opinion / J. Hart [et al.] // Ther. Adv. Hematol. - 2013. - Vol. 4. - P. 320-334.
  12. Heshmati, F. Mechanisms of action of extracorporeal photochemotherapy / F. Heshmati // Transfus. Apher. 2003. - Vol. 29. - P. 61-70.
  13. Knobler, R. Extracorporeal photopheresis: past, present, and future / R. Knobler [et al.] // R. J. Am. Acad. Dermatol. - 2009. - Vol. 61, № 4. - P. 652-665.
  14. Knobler, R. A randomized, double-blind, placebo-controlled trial of photopheresis in systemic sclerosis / R. Knobler [et al.] // J. Am. Acad. Dermatol. - 2006. - Vol. 54. - P. 793-799.
  15. Macheiner, W. Sezary syndrome and seronegative polyarthritis: treatment with extracorporeal photochemotherapy / W. Macheiner [et al.] // J. Am. Acad. Dermatol. - 2003. - Vol. 48. - P. 220-226.
  16. Marques, M. Photopheresis in solid organ transplant rejection / M. Marques [et al.] // J. Clin. Apher. - 2006. - Vol. 21. - P. 72-77.
  17. Marques, M. Update on extracorporeal photopheresis in heart and lung transplantation / M. Marques [et al.] // J. Clin. Apher. - 2011. - Vol. 26. - P.146-151.
  18. Marshall, S. Technology insight: ECP for the treatment of GvHD- can we offer selective immune control without generalized immunosuppression? / S. Marshall [et al.] // Nat. Clin. Pract. Oncol. - 2006. - Vol. 3. - P. 302-314.
  19. McKenna, K. Evidence-based practice of photopheresis 1987- 2001: a report of a workshop of the British Photodermatology Group and the U.K. Skin Lymphoma Group / K. McKenna [et al.] // British Journal of Dermatology. - 2006. - Vol. 154, № 1. - P. 7-20.
  20. Pierelli, L. Extracorporeal photopheresis for the treatment of acute and chronic graftversus-host disease in adults and children: best practice recommendations from an Italian Society of Hemapheresis and Cell Manipulation (SIdEM) and Italian Group for Bone Marrow Transplantation (GITMO) consensus process / L. Pierelli [et al.] // Transfusion - 2013. - Vol. 53. - P. 2340-2352.
  21. Saraceno, R. Therapeutic options in an immunocompromised patient with pemphigus vulgaris: potential interest of plasmapheresis and extracorporeal photochemotherapy / R. Saraceno [et al.] // Eur. J. Dermatol. - 2008. - Vol. 18. - P. 354-356.
  22. Schmid, D. T-cell death, phosphatidylserine exposure and reduced proliferation rate to validate extracorporeal photochemotherapy / D. Schmid [et al.] // Vox. Sang. - 2015. - Vol. 108, № 1. - P. 82-88.
  23. Taverna, F. Biological quality control for extracorporeal photochemotherapy: Assessing mononuclear cell apoptosis levels in ECP bags of chronic GvHD patients / F. Taverna [et al.] // J. Clin. Apher. 2015. - Vol. 30, № 6. - P. 162-170.
  24. Urbani, L. The use of extracorporeal photopheresis for allograft rejection in liver transplant recipients / L. Urbani [et al.] // Transplant. Proc. - 2004. - Vol. 36. - P. 3068-3070.
  25. Urbani, L. A novel immunosuppressive strategy combined with preemptive antiviral therapy improves the eighteen-month mortality in HCV recipients transplanted with aged livers / L. Urbani [et al.] // Transplantation. - 2008. - Vol. 86. - P. 1666-1671.
  26. Voss, C. Extending the horizon for cell-based immunotherapy by understanding the mechanisms of action of photopheresis / C. Voss [et al.] // Transfus. Med. Rev. - 2010. - Vol. 24, № 1. - P. 22-32.
  27. Yakut, E. Extracorporeal photopheresis promotes IL-1× production / E. Yakut [et al.] // J. Immunol. 2015. - Vol. 194, № 6. - P. 2569-2577.

Copyright (c) 2018 Manuilov A.S., Kudryavtsev I.V., Serebriakova M.K., Trulev A.S., Bardakov S.N., Apchel A.V., Belskih A.N., Zakharov M.V., Tishko V.V., Sokolov A.A., Vasylieva I.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies