Development of an Immunosorbent for Solid-Phase NMR-Based Assay
- Авторы: Khramtsov P.V.1,2, Kropaneva M.D.1, Bochkova M.S.1, Timganova V.P.1, Zamorina S.A.1,2, Rayev M.B.1,2
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Учреждения:
- Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences
- Perm State National Research University
- Выпуск: Том 484, № 1 (2019)
- Страницы: 69-72
- Раздел: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212791
- DOI: https://doi.org/10.1134/S1607672919010174
- ID: 212791
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Аннотация
The conditions for constructing an immunosorbent reagent for solid-phase NMR analysis were optimized. For this purpose, we increased the area of the sensitized portion of the membrane to fit the relaxometer coil size and added the agent sorption buffer. This provided the penetration of the anti-ligand molecules into the membrane thickness and their uniform distribution.
Об авторах
P. Khramtsov
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Автор, ответственный за переписку.
Email: khramtsovpavel@yandex.ru
Россия, Perm, 614081; Perm,
614600
M. Kropaneva
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Россия, Perm, 614081
M. Bochkova
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Россия, Perm, 614081
V. Timganova
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Россия, Perm, 614081
S. Zamorina
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Email: khramtsovpavel@yandex.ru
Россия, Perm, 614081; Perm,
614600
M. Rayev
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Email: khramtsovpavel@yandex.ru
Россия, Perm, 614081; Perm,
614600
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