Development of an Immunosorbent for Solid-Phase NMR-Based Assay
- Autores: Khramtsov P.V.1,2, Kropaneva M.D.1, Bochkova M.S.1, Timganova V.P.1, Zamorina S.A.1,2, Rayev M.B.1,2
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Afiliações:
- Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences
- Perm State National Research University
- Edição: Volume 484, Nº 1 (2019)
- Páginas: 69-72
- Seção: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212791
- DOI: https://doi.org/10.1134/S1607672919010174
- ID: 212791
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Resumo
The conditions for constructing an immunosorbent reagent for solid-phase NMR analysis were optimized. For this purpose, we increased the area of the sensitized portion of the membrane to fit the relaxometer coil size and added the agent sorption buffer. This provided the penetration of the anti-ligand molecules into the membrane thickness and their uniform distribution.
Sobre autores
P. Khramtsov
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Autor responsável pela correspondência
Email: khramtsovpavel@yandex.ru
Rússia, Perm, 614081; Perm,
614600
M. Kropaneva
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Rússia, Perm, 614081
M. Bochkova
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Rússia, Perm, 614081
V. Timganova
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Rússia, Perm, 614081
S. Zamorina
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Email: khramtsovpavel@yandex.ru
Rússia, Perm, 614081; Perm,
614600
M. Rayev
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Email: khramtsovpavel@yandex.ru
Rússia, Perm, 614081; Perm,
614600
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