Stabilization of Butyrylcholinesterase by the Entrapment into the Natural Polymer-Based Gels
- Авторы: Lonshakova-Mukina V.I.1, Esimbekova E.N.1,2, Kratasyuk V.A.1,2
-
Учреждения:
- Siberian Federal University
- Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Federal Research Center, Krasnoyarsk Research Center
- Выпуск: Том 479, № 1 (2018)
- Страницы: 98-100
- Раздел: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212179
- DOI: https://doi.org/10.1134/S1607672918020126
- ID: 212179
Цитировать
Аннотация
A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the procedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel.
Об авторах
V. Lonshakova-Mukina
Siberian Federal University
Email: esimbekova@yandex.ru
Россия, Krasnoyarsk, 660041
E. Esimbekova
Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Federal Research Center, Krasnoyarsk Research Center
Автор, ответственный за переписку.
Email: esimbekova@yandex.ru
Россия, Krasnoyarsk, 660041; Krasnoyarsk, 660036
V. Kratasyuk
Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Federal Research Center, Krasnoyarsk Research Center
Email: esimbekova@yandex.ru
Россия, Krasnoyarsk, 660041; Krasnoyarsk, 660036
Дополнительные файлы
