Stabilization of Butyrylcholinesterase by the Entrapment into the Natural Polymer-Based Gels


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A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the procedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel.

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V. Lonshakova-Mukina

Siberian Federal University

Email: esimbekova@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660041

E. Esimbekova

Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Federal Research Center, Krasnoyarsk Research Center

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Email: esimbekova@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660041; Krasnoyarsk, 660036

V. Kratasyuk

Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Federal Research Center, Krasnoyarsk Research Center

Email: esimbekova@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660041; Krasnoyarsk, 660036

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