Email this article Expression of senescence-associated genes in multipotent mesenchymal stromal cells during long-term cultivation at various hypoxic levels
- Authors: Lobanova M.V.1, Ratushnyy A.Y.1, Buravkova L.B.1
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Affiliations:
- Institute of Biomedical Problems
- Issue: Vol 470, No 1 (2016)
- Pages: 326-328
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211536
- DOI: https://doi.org/10.1134/S1607672916050057
- ID: 211536
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Abstract
The expression of several genes which functions are associated with cellular senescence was analyzed in multipotent mesenchymal stromal cells during long-term cultivation at different oxygen levels (20, 5, and 1%) using the RT² Profiler™ PCR Array Human Cellular Senescence system (Qiagen, United States). It was established that replicative senescence processes develop most actively in the cells cultured under the standard conditions (20% O2). The most significant changes were observed in the expression of CCND1, ID1, IGF1, PIK3CA, and SERPINE1 genes.
About the authors
M. V. Lobanova
Institute of Biomedical Problems
Author for correspondence.
Email: pogodina_m@mail.ru
Russian Federation, Khoroshevskoe sh. 76-a, Moscow, 123007
A. Y. Ratushnyy
Institute of Biomedical Problems
Email: pogodina_m@mail.ru
Russian Federation, Khoroshevskoe sh. 76-a, Moscow, 123007
L. B. Buravkova
Institute of Biomedical Problems
Email: pogodina_m@mail.ru
Russian Federation, Khoroshevskoe sh. 76-a, Moscow, 123007
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