Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast

Аннотация

A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.