Enviar artigo por via de e-mail Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast
- Autores: Trubnikova E.V.1, Isakova E.P.2, Deryabina Y.I.2, Nikolaev A.V.3
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Afiliações:
- Kursk State University
- Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
- Schmidt Joint Institute of Physics of the Earth
- Edição: Volume 468, Nº 1 (2016)
- Páginas: 190-192
- Seção: Biochemistry, Biophysics and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211217
- DOI: https://doi.org/10.1134/S160767291603008X
- ID: 211217
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Resumo
A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.
Sobre autores
E. Trubnikova
Kursk State University
Email: elen_iss@mail.ru
Rússia, Kursk
E. Isakova
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Autor responsável pela correspondência
Email: elen_iss@mail.ru
Rússia, Leninskii pr. 33, Moscow, 119071
Yu. Deryabina
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Email: elen_iss@mail.ru
Rússia, Leninskii pr. 33, Moscow, 119071
A. Nikolaev
Schmidt Joint Institute of Physics of the Earth
Email: elen_iss@mail.ru
Rússia, ul. Bol’shaya Gruzinskaya 10, Moscow, 123810
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