Email this article Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast
- Authors: Trubnikova E.V.1, Isakova E.P.2, Deryabina Y.I.2, Nikolaev A.V.3
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Affiliations:
- Kursk State University
- Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
- Schmidt Joint Institute of Physics of the Earth
- Issue: Vol 468, No 1 (2016)
- Pages: 190-192
- Section: Biochemistry, Biophysics and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211217
- DOI: https://doi.org/10.1134/S160767291603008X
- ID: 211217
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Abstract
A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.
About the authors
E. V. Trubnikova
Kursk State University
Email: elen_iss@mail.ru
Russian Federation, Kursk
E. P. Isakova
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Author for correspondence.
Email: elen_iss@mail.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071
Yu. I. Deryabina
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology,”
Email: elen_iss@mail.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071
A. V. Nikolaev
Schmidt Joint Institute of Physics of the Earth
Email: elen_iss@mail.ru
Russian Federation, ul. Bol’shaya Gruzinskaya 10, Moscow, 123810
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