Influence of dicarbonyls on kinetic characteristics of glutathione peroxidase
- 作者: Lankin V.Z.1, Shumaev K.B.1,2, Tikhaze A.K.1, Kurganov B.I.2
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隶属关系:
- Russian Cardiology Research and Production Center
- Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology”
- 期: 卷 475, 编号 1 (2017)
- 页面: 287-290
- 栏目: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211960
- DOI: https://doi.org/10.1134/S1607672917040123
- ID: 211960
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详细
Se-containing glutathione peroxidase (GSH-Px) is one of the key enzymes of the body’s antioxidant system. The kinetic characteristics of GSH-Px (substrate is tert-butyl hydroperoxide) after modification of the enzyme by various concentrations of natural dicarbonyls (glyoxal, methylglyoxal, malonic dialdehyde) were studied. It was shown that dicarbonyls affected both Km and Vmax for GSH-Px. It is suggested that the effect of various dicarbonyls on GSH-Px depends on the molecular mechanisms of their interaction with the amino acid residues of the enzyme.
作者简介
V. Lankin
Russian Cardiology Research and Production Center
编辑信件的主要联系方式.
Email: lankin941@mail.ru
俄罗斯联邦, Moscow, 121552
K. Shumaev
Russian Cardiology Research and Production Center; Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology”
Email: lankin941@mail.ru
俄罗斯联邦, Moscow, 121552; Moscow, 119071
A. Tikhaze
Russian Cardiology Research and Production Center
Email: lankin941@mail.ru
俄罗斯联邦, Moscow, 121552
B. Kurganov
Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology”
Email: lankin941@mail.ru
俄罗斯联邦, Moscow, 119071
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