ANALYSIS OF THE INFLUENCE OF GENETIC FACTORS OF HEPATITIS C VIRUS AND GENE POLYMORPHISM IN INFECTED PATIENTS ON THE DEVELOPMENT OF LIVER FIBROSIS


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Abstract

The purpose of the study - to reveal the dependence of the rate of development of liver fibrosis (LF) on genetic factors of hepatitis C virus (HCV) and single nucleotide polymorphisms (SNPs) of the genes of infected people. In the three groups of patients with different rates of development of LF, HCV subtype, viral load, the number of quasi- variant forms and the presence of intergenotype recombinantion, SNPs of human cytokine genes (IL-1ß, IL-6, IL-10, IL-28B, TNF-a , TGF-ß1), hereditary hemochromatosis (HFE), genes involved in the development of endothelial dysfunction (eNOS) and oxidative stress (CYBA) were analyzed. In all groups of patients with HCV subtype 1b prevailed. In the groups with moderate and fast speed of development of LF viral load was revealed to be higher than in the group with the slow development of fibrosis. The number of genetic variants within E2 protein zone in the latter group of patients is characterized to be more than in other groups. Recombinant HCV RNA (2k/1b) was found in the groups with moderate and rapid development of LF. SNP analysis of genes of patients showed a statistically significant relationship between the rapid formation of LF and allelic variants of IL-1ß (-511 TT), IL-10 (-1082 AA), TNF-a (-238 GA or AA) and HFE (S282Y or Y282Y )genes. In other SNPs of analyzed gene there no association with speed of LF was found.

About the authors

L. I. Nikolaeva

Federal State Budgetary Institution D.I. Ivanovsky Institute of Virology of the Ministry of Health and Social Development of the Russian Federation

Email: L.I.Nikolaeva@mail.ru
д-р биол. наук, вед. науч. сотр. лаб. генно-инженерных препаратов Moscow, Russian Federation, 16, Ulitsa Gamalei, 123098

A. V. Kolotvin

Federal State Budgetary Institution D.I. Ivanovsky Institute of Virology of the Ministry of Health and Social Development of the Russian Federation

науч. сотр. МГУ им. М.В. Ломоносова, ГУНУ фак-т фундаментальной медицины, НИИ вирусологии им. Д.И. Ивановского, лаб. генно-инженерных препаратов Moscow, Russian Federation, 16, Ulitsa Gamalei, 123098

L. M. Samokhodskaya

Federal State Educational Institution ofHigher Professional Education M.V Lomonosov, Moscow State University

канд. мед. наук; доц., ст. науч. сотр. МГУ им. М. В. Ломоносова, ГУНУ фак. фундаментальной медицины, лаб. генных и клеточных технологий 1, Vorobyovy gory, Moscow, Russian Federation, 119991

G. V. Sapronov

State Budgetary Educational Institution of Additional Professioinal Education Russian Medical Academy of Postgraduate Education

канд. мед. наук, доцент каф. инфекционных болезней ГБОУ ДПО РМАПО 2/1, Ul. Barrikadnaya, Moscow, Russian Federation, 123995

V. V. Makashova

Federal Budget Institution of Science Central Research Institute of Epidemiology of The Federal Service on Customers' Rights Protection and Human Well-being Surveillance

д-р мед. наук, проф., вед. науч. сотр ЦНИИ эпидемиологии, клинич. отд-ния инфек. патологии 3a, Ul. Novogireevskaya, Moscow, Russian Federation, 111123

E. I. Samokhvalov

Federal State Budgetary Institution D.I. Ivanovsky Institute of Virology of the Ministry of Health and Social Development of the Russian Federation

канд. мед. наук, вед. науч. сотр. НИИ вирусологии им. Д.И. Ивановского, лаб. экологии вирусов Moscow, Russian Federation, 16, Ulitsa Gamalei, 123098

S. V. Al’khovsky

Federal State Budgetary Institution D.I. Ivanovsky Institute of Virology of the Ministry of Health and Social Development of the Russian Federation

канд. биол. наук, зав. лаб. НИИ вирусологии им. Д.И. Ивановского, лаб. биотехнологии Moscow, Russian Federation, 16, Ulitsa Gamalei, 123098

A. E. Grishechkin

Federal State Budgetary Institution D.I. Ivanovsky Institute of Virology of the Ministry of Health and Social Development of the Russian Federation

науч. сотр. НИИ вирусологии им. Д.И. Ивановского, лаб. генно-инженерных препаратов Moscow, Russian Federation, 16, Ulitsa Gamalei, 123098

N. M. Belyaeva

State Budgetary Educational Institution of Additional Professioinal Education Russian Medical Academy of Postgraduate Education

д-р мед. наук, проф., зав. каф. инфекционных болезней ГБОУ ДПО РМАПО 2/1, Ul. Barrikadnaya, Moscow, Russian Federation, 123995

R. A. Gibadulin

Federal State Budgetary Institution D.I. Ivanovsky Institute of Virology of the Ministry of Health and Social Development of the Russian Federation

д-р мед. наук, проф., зав. лаб. НИИ вирусологии им. Д.И. Ивановского, лаб. генно-инженерных препаратов. Moscow, Russian Federation, 16, Ulitsa Gamalei, 123098

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