Method of DNA-comet assay in the diagnosis of liver fibrosis in patients with chronic viral hepatitis C


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Abstract

In the study there were included 100 patients, which were selected in 5 group in dependence on the degree of liver fibrosis: I - F0, II - F1, III - F2, IV- F3, V - F4. Sixth (control) group was consisted of 43 healthy volunteers. The performed research has shown the relationship between the stage of fibrosis and the degree of DNA degradation in peripheral blood lymphocytes. The biggest level of DNA destruction in peripheral blood lymphocytes was established in group V patients with chronic viral hepatitis C. This allowed to consider method of DNA-comet assay as promising auxiliary method in the assessment of the formation of liver fibrosis.

About the authors

A. F Popov

Pacific State Medical University

Email: doctor.popov@mail.ru
доктор мед. наук, проф., проф. каф. инфекционных болезней 2, Ostryakova Pr., Vladivostok, Russian Federation, 690002

A. O Mikhailov

Pacific State Medical University

Email: mao1991@mail.ru
клинический интерн кафедры терапии, функциональной и ультразвуковой диагностики ФПКиППС 2, Ostryakova Pr., Vladivostok, Russian Federation, 690002

N. S Ivanova

Pacific State Medical University

кандидат химических наук, доц., заведующая кафедрой общей и биологической химии 2, Ostryakova Pr., Vladivostok, Russian Federation, 690002

A. I Simakova

Pacific State Medical University

Email: anna-inf@yandex.ru
доктор медицинских наук, доцент, заведующая кафедрой инфекционных болезней 2, Ostryakova Pr., Vladivostok, Russian Federation, 690002

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