MORPHOLOGICAL BASIS OF AUTOIMMUNE MANIFESTATIONS IN VIRAL HEPATITISES


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Abstract

The purpose of the study - to study morphological basis of extrahepatic lesions and autoimmune manifestations in chronic viral hepatitises Methods of performance. By indirect immunofluorescence ASMA, AMA, ANA, by enzyme immunoassay - the Anti-LKM-1 were determined. Cryoglobulins (CG) have been revealed with the use of qualitative visualization method, rheumatoid factor (RF) was determined in serum by semiquantitative latex agglutination on a slide. Patients underwent liver biopsy. A punction liver biopsy according to Mengini was performed with the subsequent histologic and morphological research of obtained liver bioptates with the use of an immunohistochemKal and immunocytochemical methods. Results. In patients with chronic viral hepatitis autoimmune manifestations (RF, CG, ASMA, ANA, AMA, anti-LKM-1) were identified at the same rate regardless of the degree of histological activity. In the liver bioptate in patients with chronic viral hepatitises the complex of regulatory T cells (CD4 + CD25 +), which indicates to the presence of morphological and functional basis for the formation of autoimmune manifestations against persistent viral infection was detected. Conclusion. Identified by ourselves autoimmune manifestations in the blood serum be embodied in the morphological changes of the liver tissue, at that the morphological changes characteristic of autoimmune hepatitises, are observed mainly in patients with chronic viral hepatitis B and C, with moderate and high histological activity.

About the authors

K. T. Umbetova

State Budgetary Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health care and Social Development

Email: karmasara@m-box.ru
д-р мед. наук, проф. каф. инфекционных болезней медикопрофилактического фак. Первого МГМУ Moscow, Russian Federation, 8-2, Trubetskaya street, Moscow, Russia, 119991

E. V. Volchkova

State Budgetary Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health care and Social Development

Email: antononina@rambler.ru
д-р мед. наук, проф., зав. каф. инфекционных болезней медико-профилактического фак. Первого МГМУ им. И.М. Сеченова Moscow, Russian Federation, 8-2, Trubetskaya street, Moscow, Russia, 119991

M. V. Kiselevsky

Federal State Budgetary Institute N. N. Blokhin Cancer Research Center of the Russian Academy of Medical Sciences

д-р мед. наук, проф., член-кор. РАМН, зав. лаб. клеточного иммунитета РОНЦ им. Н.Н. Блохина РАМН 24, Kashirskoye Shosse, Moscow, Russian Federation, 115478

Yu. G. Parkhomenko

State Treasury Institution of Healthcare “Infectious Clinical Hospital № 2” of the Moscow Department of Healthcare

д-р мед. наук, проф., зав. патологоанатомическим отд-нием ИКБ № 2 15, Vos’maya Ulitsa Sokolinoy gory, Moscow, Russian Federation, 105275

L. I. Shuba

State Budgetary Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health care and Social Development

канд. мед. наук, доцент каф. инфекционных болезней медико-профилактического фак. Первого МГМУ им. И.М. Сеченова Moscow, Russian Federation, 8-2, Trubetskaya street, Moscow, Russia, 119991

S. G. Pak

State Budgetary Educational Institution of Higher Professional Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health care and Social Development

д-р мед. наук, проф., член-кор. РАМН, почетный зав. каф. инфекционных болезней медико-профилактического фак. Первого МГМУ им. И.М. Сеченова. Moscow, Russian Federation, 8-2, Trubetskaya street, Moscow, Russia, 119991

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