Laboratory diagnosis of Yersinia pseudotuberculosis and Yersinia enterocolitica

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Abstract

Enteropathogenic species Yersinia pseudotuberculosis and Yersinia enterocolitica are of great importance worldwide due to their ability to survive and preserve pathogenic properties in biotic and abiotic environments. They can cause sporadic and flare-up diseases. Therefore, it is extremely important to use modern methods of diagnosing diseases caused by these pathogens.

The article analyzes the methods of laboratory diagnostics of enteropathogenic Yersinia (Y. pseudotuberculosis and Y. enterocolitica), including bacteriological, serological and molecular genetic. Modern data on the use of the polymerase chain reaction method, mass spectrometry (МALDI-TOF MS), dot immunoassay are presented. The scheme of the optimal protocol for the detection and identification of Yersinia is given.

The combination of classical microbiological and molecular genetic methods quickly determines the presence or absence of a pathogen in a sample, which allows the bacteriological study to be purposefully used to verify the clinical diagnosis, confirm transmission factors and plan anti-epidemic measures.

About the authors

Margarita V. Chesnokova

Irkutsk Antiplague Research Institute of Siberia and Far East

Author for correspondence.
Email: mar_chumin@mail.ru
ORCID iD: 0000-0001-5489-9363
SPIN-code: 2005-4321

MD, Dr. Sci. (Med), Professor

Russian Federation, 78, Trilissera street, Irkutsk, 664047

Valeriy T. Klimov

Irkutsk Antiplague Research Institute of Siberia and Far East

Email: 41vklimov@yandex.ru
ORCID iD: 0000-0003-0036-0017
SPIN-code: 1382-9190

MD, Cand. Sci. (Med), Senior Research Assistant

Russian Federation, 78, Trilissera street, Irkutsk, 664047

Tatiana V. Karimova

Administration of Rospotrebnadzor in Novosibirsk Region

Email: tatianakarimova357@gmail.com
ORCID iD: 0000-0001-9099-3621
SPIN-code: 7101-4016

MD, Cand. Sci. (Med.)

Russian Federation, Novosibirsk

Tatiana Yu. Zagoskina

Irkutsk Antiplague Research Institute of Siberia and Far East

Email: t_y_z_@mail.ru
ORCID iD: 0000-0002-9302-7143
SPIN-code: 5891-7130

MD, Dr. Sci. (Med.)

Russian Federation, 78, Trilissera street, Irkutsk, 664047

Alexander L. Panin

Saint-Petersburg Pasteur Institute

Email: alp.1952@mail.ru
ORCID iD: 0000-0001-6411-0274
SPIN-code: 4690-3587

Junior Research Assistant

Russian Federation, Saint Petersburg

References

  1. Adeolu M, Alnajar S, Naushad S, Gupta RS. Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Microbiol. 2016;66(12):5575−5599. doi: 10.1099/ijsem.0.001485
  2. Somova LM, Andriukov BG, Timchenko NF, Psareva EK. Pseudotuberculosis as a persistent infection: etiopathogenetic prerequisites. J Microbiol Epidemiology Immunobiology. 2019;(2):110−119. (In Russ). doi: 10.36233/0372-9311-2019-2-110-119
  3. Savin C, Frangeul L, Ma L, et al. Draft genome sequence of a clinical strain of Yersinia enterocolitica (ip10393) of bioserotype 4/O:3 from France. Genome Announc. 2013;1(1):e00150-12. doi: 10.1128/genomeA.00150-12
  4. Shurygina IA, Chesnokova MV, Klimov VT, et al. Pseudotuberculosis. Novosibirsk: Nauka; 2003. 320 p. (In Russ).
  5. Fredriksson-Ahomaa M, Korkeala H. Low occurrence of pathogenic Yersinia enterocolitica in clinical, food, and environmental samples: a methodological problem. Clin Microbiol Rev. 2003; 16(2):220−229. doi: 10.1128/CMR.16.2.220-229.2003
  6. Black RE, Jackson RJ, Tsai T, et al. Epidemic Yersinia enterocolitica infection due to contaminated chocolate milk. N Engl J Med. 1978;298(2):76−79. doi: 10.1056/NEJM197801122980204
  7. Tacket CO, Ballard J, Harris N, et al. An outbreak of Yersinia enterocolitica infections caused by contaminated tofu (soybean curd). Am J Epidemiol. 1985;121(5):705−711. doi: 10.1093/aje/121.5.705
  8. Abraham M, Pai M, Kang G, et al. An outbreak of food poisoning in Tamil Nadu associated with Yersinia enterocolitica. Indian J Med Res. 1997;106:465−468.
  9. Swaminathan B, Harmon MC, Mehlman IJ. Yersinia enterocolitica. J Appl Bacteriol. 1982;52(2):151−183. doi: 10.1111/j.1365-2672.1982.tb04838.x
  10. Chesnokova MV. Pseudotuberculosis in Siberia: Theoretical and applied aspects of epidemiology, laboratory diagnostics and prevention [dissertation abstract]. Irkutsk; 2004. 42 p. (In Russ).
  11. Martínez PO, Fredriksson-Ahomaa M, Sokolova Y, et al. Prevalence of enteropathogenic Yersinia in Estonian, Latvian, and Russian (Leningrad region) pigs. Foodborne Pathog Dis. 2009;6(6):719−724. doi: 10.1089/fpd.2008.0251
  12. Fukushima H, Matsuda Y, Seki R, et al. Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains. J Clin Microbiol. 2001;39(10):3541−3547. doi: 10.1128/JCM.39.10.3541-3547.2001
  13. Niléhn B. Studies on Yersinia enterocolitica with special reference to bacterial diagnosis and occurrence in human acute enteric disease, 1969. APMIS. 2007;115(5):578−588; discussion 589-591. doi: 10.1111/j.1600-0463.2007.apm_725.x
  14. Landgraf M, Iaria ST, Falcão DP. An improved enrichment procedure for the Isolation of Yersinia enterocolitica and related species from milk. J Food Prot. 1993;56(5):447−450. doi: 10.4315/0362-028X-56.5.447
  15. Fondrevez M, Labbé A, Houard E, et al. A simplified method for detecting pathogenic Yersinia enterocolitica in slaughtered pig tonsils. J Microbiol Methods. 2010;83(2):244−249. doi: 10.1016/j.mimet.2010.09.012
  16. Fredriksson-Ahomaa M. Isolation of enteropathogenic Yersinia from non-human sources. Adv Exp Med Biol. 2012;954:97−105. doi: 10.1007/978-1-4614-3561-7_12
  17. Carniel E. The Yersinia high-pathogenicity island. Int Microbiol. 1999;2(3):161−167.
  18. Fukushima H, Gomyoda M, Tsubokura M, Aleksić S. Isolation of Yersinia pseudotuberculosis from river waters in Japan and Germany using direct KOH and HeLa cell treatments. Zentralbl Bakteriol. 1995;282(1):40−49. doi: 10.1016/s0934-8840(11)80795-4
  19. Koujitani E, Horisaka T, Nomura Y, et al. Immuno-magnetic separation and agar layer methods for the isolation of freeze-injured Yersinia enterocolitica O:8 from water. J Vet Med Sci. 2006;68(3):195−199. doi: 10.1292/jvms.68.195
  20. Fukushima H, Shimizu S, Inatsu Y. Yersinia enterocolitica and Yersinia pseudotuberculosis detection in foods. J Pathog. 2011;2011:735308. doi: 10.4061/2011/735308
  21. Aulisio CC, Mehlman IJ, Sanders AC. Alkali method for rapid recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods. Appl Environ Microbiol. 1980;39(1):135−140. doi: 10.1128/aem.39.1.135-140.1980
  22. Fukushima H, Tsubokura M, Otsuki K, et al. Epidemiological study of Yersinia enterocolitica and Yersinia pseudotuberculosis infections in Shimane Prefecture, Japan. Zentralbl Bakteriol Mikrobiol Hyg B. 1985;180(5-6):515−527.
  23. Weagant SD. A new chromogenic agar medium for detection of potentially virulent Yersinia enterocolitica. J Microbiol Methods. 2008;72(2):185−190. doi: 10.1016/j.mimet.2007.11.019
  24. Tseneva GY, Messorosh VG, Kulyashova LB, et al. The effectiveness of the use of nutrient media for the isolation of yersinia. Сlin Lab Diagnostics. 1993;(3):73−74. (In Russ).
  25. Ito T, Suzuki T, Kawase J, et al. Yersinia enterocolitica bacteremia and enterocolitis in a previously healthy 20-month-old girl. J Infect Chemother. 2012;18(5):756−759. doi: 10.1007/s10156-011-0353-8
  26. Van Damme I, Berkvens D, de Zutter L. Effect of sampling and short isolation methodologies on the recovery of human pathogenic Yersinia enterocolitica from pig tonsils. Foodborne Pathog Dis. 2012;9(7):600−606. doi: 10.1089/fpd.2012.1128
  27. Niskanen T, Laukkanen R, Murros A, et al. Characterisation of non-pathogenic Yersinia pseudotuberculosis-like strains isolated from food and environmental samples. Int J Food Microbiol. 2009;129(2):150−156. doi: 10.1016/j.ijfoodmicro.2008.11.015
  28. Sharma NK, Doyle PW, Gerbasi SA, Jessop JH. Identification of Yersinia species by the API 20E. J Clin Microbiol. 1990;28(6):1443−1444. doi: 10.1128/jcm.28.6.1443-1444.1990
  29. Linde HJ, Neubauer H, Meyer H, et al. Identification of Yersinia species by the Vitek GNI card. J Clin Microbiol. 1999;37(1):211−214. doi: 10.1128/JCM.37.1.211-214.1999
  30. Bogdanovich T, Carniel E, Fukushima H, Skurnik M. Use of O-antigen gene cluster-specific PCRs for the identification and O-genotyping of Yersinia pseudotuberculosis and Yersinia pestis. J Clin Microbiol. 2003;41(11):5103−5112. doi: 10.1128/JCM.41.11.5103-5112.2003
  31. Laird WJ, Cavanaugh DC. Correlation of autoagglutination and virulence of Yersiniae. J Clin Microbiol. 1980;11(4):430−432. doi: 10.1128/jcm.11.4.430-432.1980
  32. Bhaduri S, Turner-Jones C, Lachica RV. Convenient agarose medium for simultaneous determination of the low-calcium response and Congo red binding by virulent strains of Yersinia enterocolitica. J Clin Microbiol. 1991;29(10):2341−2344. doi: 10.1128/jcm.29.10.2341-2344.1991
  33. Zink DL, Feeley JC, Wells JG, et al. Plasmid-mediated tissue invasiveness in Yersinia enterocolitica. Nature. 1980;283(5743): 224−226. doi: 10.1038/283224a0
  34. Kapperud G. Yersinia enterocolitica in food hygiene. Int J Food Microbiol. 1991;12(1):53−65. doi: 10.1016/0168-1605(91)90047-s
  35. Kapperud G, Vardund T. Detection of pathogenic Yersinia enterocolitica in food, water, and feces by nested polymerase chain reactions and immunomagnetic separation. Contrib Microbiol Immunol. 1995;13:130−133.
  36. Skurnik M, Wolf-Watz H. Analysis of the YopA gene encoding the Yop1 virulence determinants of Yersinia spp. Mol Microbiol. 1989;3(4):517−529. doi: 10.1111/j.1365-2958.1989.tb00198.x
  37. Wang RF, Cao WW, Cerniglia CE. A universal protocol for PCR detection of 13 species of foodborne pathogens in foods. J Appl Microbiol. 1997;83(6):727−736. doi: 10.1046/j.1365-2672.1997.00300.x
  38. Lambertz ST, Nilsson C, Hallanvuo S. TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food. Appl Environ Microbiol. 2008;74(20):6465−6469. doi: 10.1128/AEM.01459-08
  39. Wren BW, Tabaqchali S. Detection of pathogenic Yersinia enterocolitica by the polymerase chain reaction. Lancet. 1990; 336(8716):693. doi: 10.1016/0140-6736(90)92191-j
  40. Vishnubhatla A, Fung DY, Oberst RD, et al. Rapid 5’ nuclease (TaqMan) assay for detection of virulent strains of Yersinia enterocolitica. Appl Environ Microbiol. 2000;66(9):4131−4135. doi: 10.1128/AEM.66.9.4131-4135.2000
  41. Wannet WJ, Reessink M, Brunings HA, Maas HM. Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay. J Clin Microbiol. 2001;39(12):4483−4486. doi: 10.1128/JCM.39.12.4483-4486.2001
  42. Sandery M, Stinear T, Kaucner C. Detection of pathogenic Yersinia enterocolitica in environmental waters by PCR. J Appl Bacteriol. 1996;80(3):327−332. doi: 10.1111/j.1365-2672.1996.tb03227.x
  43. Fukushima H, Tsunomori Y, Seki R. Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools. J Clin Microbiol. 2003;41(11):5134−5146. doi: 10.1128/JCM.41.11.5134-5146.2003
  44. Thisted Lambertz S, Danielsson-Tham ML. Identification and characterization of pathogenic Yersinia enterocolitica isolates by PCR and pulsed-field gel electrophoresis. Appl Environ Microbiol. 2005;71(7):3674−3681. doi: 10.1128/AEM.71.7.3674-3681.2005
  45. Rossen L, Nørskov P, Holmstrøm K, Rasmussen OF. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol. 1992;17(1):37−45. doi: 10.1016/0168-1605(92)90017-w
  46. Stephan R, Cernela N, Ziegler D, et al. Rapid species identification and subtyping of Yersinia enterocolitica by MALDI-TOF mass spectrometry. J Microbiol Methods. 2011;87(2):150−153. doi: 10.1016/j.mimet.2011.08.016

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2. Fig. Scheme of Yersinia’s detection and identification. Note: ПЦР — polymerase chain reaction, DIA — Dot immunoassay, Y.ptbc — Yersinia pseudotuderculosis, Y.ent — Yersinia enterocolitica, МФА — Fluorescent antibody method, inv — invasiveness gene, HPI — High Pathogenicity Island, YAPI — Yersinia Adhesion Pathogenicity Island, pYV — Plasmid associated with Yesinia Virulence Y. pseudotuderculosis, ypm A/C — gene Y. pseudotuberculosis-derived Mitogen A/C, ail — gene of adhesion-invasion Y. enterocolitica, yst А — gene Y. enterocolitica stable Toxin A, yst В — gene Y. enterocolitica stable Toxin B, VNTR — Variable Number of Tandem Repeat, PFGA — Pulsed-Field Gel Electrophoresis.

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