Use of Specific Properties of Poly-N-vinylcaprolactam in Immunochemical Reactions

Аннотация

Principal possibility of using a smart polymer, poly-N-vinylcaprolactam, for immobilization of biopharmaceuticals (antibodies and antigens) in the course of phase transition of a soluble coil into an insoluble globule was demonstrated. A procedure for preparing a hydrosol stable at room temperature in the presence of a stabilizer was examined. The hydrosol obtained, in which the collapsed polymer with the captured antigen acts as a solid support, can be used for agglutination with a complementary antibody, i.e., the immunochemical reaction can be evaluated visually.