Mass Spectrometric Analysis of Microcystins from Cyanobacterial Biomass: Optimization of the Sample Preparation Procedure
- Authors: Chernova Е.N.1, Russkikh I.V.1, Afonina E.I.1, Keltsieva О.А.1,2, Ostrovskii V.A.1,3, Podolskaya Е.P.1,2, Zhakovskaya Z.А.1
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Affiliations:
- St. Petersburg Scientific Research Center for Ecological Safety
- Institute for Analytical Instrumentation
- St. Petersburg State Institute of Technology (Technical University)
- Issue: Vol 87, No 13 (2017)
- Pages: 3123-3132
- Section: Article
- URL: https://journals.rcsi.science/1070-3632/article/view/221879
- DOI: https://doi.org/10.1134/S1070363217130035
- ID: 221879
Cite item
Abstract
The sample preparation procedure for determination of microcystins from biomass by the LC–MS method was optimized. Treatment of freeze-dried biomass samples with acetonitrile prior to the main extraction was effectively used for decreasing the amount of protein-like matrix compounds in extracts. Use of acetonitrile–water mixtures during the main ultrasound-assisted extraction allowed increasing the total microcystins amount in the resultant extracts compared to the traditional extraction using 75% methanol.
About the authors
Е. N. Chernova
St. Petersburg Scientific Research Center for Ecological Safety
Author for correspondence.
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110
Ia. V. Russkikh
St. Petersburg Scientific Research Center for Ecological Safety
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110
E. I. Afonina
St. Petersburg Scientific Research Center for Ecological Safety
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110
О. А. Keltsieva
St. Petersburg Scientific Research Center for Ecological Safety; Institute for Analytical Instrumentation
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110; Rizhskii pr. 26, St. Petersburg, 190103
V. A. Ostrovskii
St. Petersburg Scientific Research Center for Ecological Safety; St. Petersburg State Institute of Technology (Technical University)
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110; Moskovskii pr. 26, St. Petersburg, 190013
Е. P. Podolskaya
St. Petersburg Scientific Research Center for Ecological Safety; Institute for Analytical Instrumentation
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110; Rizhskii pr. 26, St. Petersburg, 190103
Z. А. Zhakovskaya
St. Petersburg Scientific Research Center for Ecological Safety
Email: s3561389@yandex.ru
Russian Federation, ul. Korpusnaya 18, St. Petersburg, 197110