Wound Healing Effect of Autologous Stromal-Vascular Fraction Cells on Induced Damage to Skin

Abstract

A course of reparative regeneration after skin damage caused by a jagged wound under the effect of the autologous stroma-vascular fraction cell suspension has been studied. The methods for clinical observation, the studies using photo-optical biopsy instrumentation, and the macro- and microscopic morphometric measurements were used. The cell material was derived from the animal’s withers with the liposuction technique. The autologous adipose tissue was used to prepare the cell suspension containing a cell group (of fibroblasts, pericytes, acrophags, adipocytes, and endothelial cells) within the stromal vascular fraction. The obtained data indicate that the cellular product contributes to a faster wound-healing process and enhanced formation of regenerator morphologically similar to the intact skin surrounding the wound (when compared to the control). In the regenerator structure, the restored areas of the skin structure layers (epidermis, dermis, and dermal adipocyte tissue) were microscopically revealed. A small site of a scar structure tended to remain in the regenerator’s central part, which was significantly smaller than that in the analogue control. It has been concluded that the cellular products have a potential to enhance the processes of reparative regeneration and can be efficient in skin-wound healing.