Synthesis and evaluation of cytotoxicity of 6-amino-4-aryl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbonitriles
- 作者: Atapour-Mashhad H.1, Soukhtanloo M.2, Massoudi A.1, Shiri A.3, Bakavoli M.3
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隶属关系:
- Chemistry Department
- Department of Clinical Biochemistry
- Department of Chemistry, Faculty of Science
- 期: 卷 42, 编号 3 (2016)
- 页面: 316-322
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/227957
- DOI: https://doi.org/10.1134/S1068162016020047
- ID: 227957
如何引用文章
详细
Several derivatives of 6-amino-4-aryl-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbonitriles were synthesized via Biginelli type reaction and tested for their anti-proliferative activity on human breast cancer (MCF-7) and human colon carcinoma (HT29) cell lines. Malignant and non-malignant cells were cultivated in RPMI medium and incubated with different concentrations of these pyrimidines. Cell viability was evaluated by MTT assay. Apoptotic cells were determined using DAPI (4'-6-diamidino-2-phenylindole) and propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1 peak). 6-Amino-4-(4-chlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbonitrile and 6-amino-4-[4-dimethylamino)phenyl]-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbonitrile decreased the viability of MCF-7 and HT29 cells, in contrast to L929 cells. These compounds induced a sub-G1 peak inflow cytometry histograms of treated cells indicating that apoptosis is involved in their toxicity.
作者简介
Hoda Atapour-Mashhad
Chemistry Department
Email: mbakavoli@um.ac.ir
伊朗伊斯兰共和国, Tehran, 19395-4697
Mohammad Soukhtanloo
Department of Clinical Biochemistry
Email: mbakavoli@um.ac.ir
伊朗伊斯兰共和国, Mashhad
Abdolhossien Massoudi
Chemistry Department
Email: mbakavoli@um.ac.ir
伊朗伊斯兰共和国, Tehran, 19395-4697
Ali Shiri
Department of Chemistry, Faculty of Science
Email: mbakavoli@um.ac.ir
伊朗伊斯兰共和国, Mashhad, 91775-1436
Mehdi Bakavoli
Department of Chemistry, Faculty of Science
编辑信件的主要联系方式.
Email: mbakavoli@um.ac.ir
伊朗伊斯兰共和国, Mashhad, 91775-1436