Synthesis of TaqMan probes using bisphosphoramidite of cyanine dye Cy3
- 作者: Ryabinin V.1, Kostina E.1, Maksakova G.1, Sinyakov A.1
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隶属关系:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
- 期: 卷 42, 编号 1 (2016)
- 页面: 111-114
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/227819
- DOI: https://doi.org/10.1134/S1068162015050131
- ID: 227819
如何引用文章
详细
A method of the synthesis of TaqMan probes with the use of bisphosphoramidite of cyanine dye Cy3 was proposed. The synthesis results in two variants of the probes consisting of either one or two oligonucleotide residues (bearing a quencher at the 3' end) attached to the dye, with the first variant being predominant. The RT-PCR probe of the classical structure bearing one oligonucleotide residue was shown to be more efficient as compared to the probe containing two oligonucleotides.
作者简介
V. Ryabinin
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
编辑信件的主要联系方式.
Email: ryabinin@niboch.nsc.ru
俄罗斯联邦, pr. Lavrent’eva 8, Novosibirsk, 630090
E. Kostina
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: ryabinin@niboch.nsc.ru
俄罗斯联邦, pr. Lavrent’eva 8, Novosibirsk, 630090
G. Maksakova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: ryabinin@niboch.nsc.ru
俄罗斯联邦, pr. Lavrent’eva 8, Novosibirsk, 630090
A. Sinyakov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: ryabinin@niboch.nsc.ru
俄罗斯联邦, pr. Lavrent’eva 8, Novosibirsk, 630090