Comparative Analysis of Nucleotide Fluorescent Analogs for Registration of DNA Conformational Changes Induced by Interaction with Formamidopyrimidine-DNA Glycosylase Fpg
- Авторы: Kuznetsova A.1, Kladova O.1, Barthes N.2, Michel B.2, Burger A.2, Fedorova O.1,3, Kuznetsov N.1,3
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Учреждения:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis
- Department of Natural Sciences, Novosibirsk State University
- Выпуск: Том 45, № 6 (2019)
- Страницы: 591-598
- Раздел: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229262
- DOI: https://doi.org/10.1134/S1068162019060256
- ID: 229262
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Аннотация
DNA-substrates containing fluorescent DNA base analogs are widely used to study protein–nucleic acid interactions. In the case of DNA-recognizing enzymes, this approach allows one to register conformational changes in DNA during the formation of enzyme–substrate complexes. An important part of such research is the design of model DNA substrates, which includes both the photophysical properties of the fluorescent groups and their location relative to a specific recognition site, namely, in the same chain on the 5′-, 3′-side or in the complementary chain opposite the specific site. In this work, we report a comparative study of the sensitivity of various fluorescent DNA base analogs, such as 2-aminopurine (aPu), pyrrolocytosine (CPy), 1,3-diaza-2-oxophenoxazine (tCO) and 3-hydroxychromone (3HC), to conformational transformations of DNA in the process of interaction with formamidopyrimidine-DNA glycosylase (Fpg) from Escherichia coli.
Об авторах
A. Kuznetsova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: nikita.kuznetsov@niboch.nsc.ru
Россия, Novosibirsk, 630090
O. Kladova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: nikita.kuznetsov@niboch.nsc.ru
Россия, Novosibirsk, 630090
Nicolas Barthes
Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis
Email: nikita.kuznetsov@niboch.nsc.ru
Франция, Nice, CNRS, 06100
Benoit Michel
Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis
Email: nikita.kuznetsov@niboch.nsc.ru
Франция, Nice, CNRS, 06100
Alain Burger
Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis
Email: nikita.kuznetsov@niboch.nsc.ru
Франция, Nice, CNRS, 06100
O. Fedorova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Department of Natural Sciences, Novosibirsk State University
Автор, ответственный за переписку.
Email: fedorova@niboch.nsc.ru
Россия, Novosibirsk, 630090; Novosibirsk, 630090
N. Kuznetsov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Department of Natural Sciences, Novosibirsk State University
Автор, ответственный за переписку.
Email: nikita.kuznetsov@niboch.nsc.ru
Россия, Novosibirsk, 630090; Novosibirsk, 630090